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CAS RN: | Product Number: T4032
Clearing Reagent iCBiofilm-H2 [for Biofilm]
Purity:
Synonyms:
Product Documents:
Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
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8ML |
¥10,000
|
16 | It can be shipped from Saitama warehouse on the same day* | Contact Us |
* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us
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* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Size : 8 mL
Description
- Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms.
In order to observe the structures of biofilms with a thickness of more than several tens of micrometers, Clearing Reagent iCBiofilm-H2 has been developed. iCBioflim-H2 is supplied as a stock solution. Before adding to biofilm, iCBiofilm-H2 has to be diluted with deionized water to appropriate concentration (undiluted iCBiofilm-H2 may work well). By adding iCBiofilm-H2 solution to a biofilm, the biofilm becomes transparent within several seconds and easy-to-observe by light microscopy such as confocal laser scanning microscopy.
*This product was developed by Associate Prof. Sugimoto, the Jikei University School of Medicine.
Storage
- Stored at room temperature and used within 6 months.
Direction for Use
-
- Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
- After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
- Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
- Stain using appropriate fluorescence reagents.
- Dilute iCBiofilm-H2 (Product No. T4032) with deionized water to an appropriate concentration.
Recommendation ratio; iCBiofilm-H2 : water = 2 : 1 or 1 : 1
If you need the best performance of iCBioflim-H2 for a target biofilm, please consider to examine different concentrations of iCBiofilm-H2. - Remove staining reagents and add diluted or undiluted iCBiofilm-H2 solution (about 500 µL – 1 mL).
- Observe the biofilm by optical microscopy.
Related Products
- T4031 Clearing Reagent iCBiofilm-H1 [for Biofilm]
- I1275 Clearing Reagent iCBiofilm-D [for Biofilm]
Product Number | T4032 |
Grade | SU |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
Appearance | Colorless to Almost colorlessclear liquid |
Functionality test | to pass test |
Refractive index | 1.4550 to 1.4650 |
Properties (reference)
GHS
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Application
Clearing of biofilm
Reference
- Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging
Application
Microscopic observation of biofilm
References
- Light and Electron Microscopic Studies of Microorganisms Growing in Rotating Biological Contactor Biofilms
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