4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

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Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
20ML	₩97,500	≥80	14	Contact Us	Shipping Simulation

* Please contact our distributor of SEJIN CI Co., Ltd. if you would like to purchase TCI products. The above prices do not include freight cost, customs charge and other charges to the destination. SEJIN CI Co., Ltd. (Phone: 02-2655-2480 / email:  sales@sejinci.co.kr)
* The storage conditions are subject to change without notice.

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Product Information

• Volume : 20 mL

Description

• This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
  SDS-PAGE Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system.
  Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.

Direction for Use

• Preparation of non-reducing sample
  A protein sample is mixed with the 4X SDS-PAGE sample buffer (3:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
• Preparation of reducing sample (reducing with 2-Mercaptoethanol)
  Add 20 μL of 2-Mercaptoethanol per 80 μL of 4X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (3:1) and heating to 95–100°C for 5 minutes. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
  *Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.

Storage

• Store at room temperature.

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