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CAS RN: | 제품번호: R0230
rPSL1a-Biotin [for Siaα(2-6)Gal]
순도/분석 방법:
- rPSL1a-Biotin Conjugate
- Recombinant Polyporus squamosus lectin (= rPSL1a)-Biotin Conjugate
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제품보충정보:
Product Information
Product code: R0230
Product name: rPSL1a-Biotin [for Sia α(2-6)Gal]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant PSL1a, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >31 kDa
Preservative : None
Stabilizer: None
Description
The rPSL1a is a carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. The rPSL1a gene product is expressed in Escherichia coli. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.). Consequently, rPSL1a exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rPSL1a-Biotin shows significant interaction with human transferrin (hTF) possessing [Siaα(2-6)Gal] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rPSL1a-Biotin specifically binds to α(2-6)Sia-terminated hTF but not to unsialylated glycoproteins such as asialo hTF, agalacto hTF and bovine RNase B.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
제품번호 | R0230 |
물리적 상태 (20 ℃) | Liquid |
보관 조건 ![]() |
Frozen (-20°C) |
피해야 할 조건 | Heat Sensitive |
Appearance | Colorless to Almost colorless clear liquid |
Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
Suitability for use in ELISA tests | min. 1.0 (1.0 micro g/mL, OD450) |
Molecular weight | 25 to 40 kDa |
HS 번호* | 3504.00-000 |
References
- Purification and characterization of a Neu5Acα2-6Galβ1-4Glc/GlcNAc-specific lectin from the fruiting body of the polypore mushroom Polyporus squamosus
- Cloning, expression in Escherichia coli and characterization of the recombinant Neu5Acα2,6Galβ1,4GlcNAc-specific high-affinity lectin and its mutants from the mushroom Polyporus squamosus
- Structure and binding analysis of Polyporus squamosus lectin in complex with the Neu5Acα2-6Galβ1-4GlcNAc human-type influenza receptor
SDS
요청한 SDS를 사용할 수 없습니다.
번거롭게 해드려 죄송하지만 이 양식 보다 문의 해주십시오.
규격표
시험성적서, 각종 증명서
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분석 차트
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