CAS RN: | 제품번호: T3940

Tissue-Clearing Reagent iTOMEI-D [for Plants]

순도/분석 방법:

동의어의:

제품문서:

포장단위	가격	당일출고	2-3일 이내 출고	추가로 필요한 경우	출하 정보
5ML	₩56,600	4	0	문의	출하 시뮬레이션
25ML	₩171,600	31	0	문의	출하 시뮬레이션

•본 제품의 재고는 일본 본사의 재고입니다. 주문시, 5일안에 실험실까지 도착됩니다.
•본건의 원가격은 한국 대리점의 예상 판매가격입니다.자세한 정보가 필요하시면 연락해 주십시오.( SEJIN CI Co., Ltd. (한국총대리점) 전화 : 02-2655-2480 이메일 : sales@sejinci.co.kr)
•보관 조건은 예고없이 변경 될 수 있습니다. 제품 보관 조건의 최신 자료는 홈페이지에 기재되어 있으니 양해 부탁드립니다.

제품보충정보:

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.

Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.

Storage

The product should be stored at 4 °C.

Directions for Use

Required reagents;

Paraformaldehyde (PFA)
PBS
Stains* (option)
Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

Tissue fixation
Reagent : 1% PFA in PBS
Time : 1hr
PBS Wash ×3
Decoloring
Reagent : iTOMEI-D (TCI Product No. T3940)
Time : 24hr
PBS Wash ×3
(Optional process Staining*, PBS Wash ×3)
Clearing (RI matching)
Reagent : Mounting solution**
Time : Operation time is determined by the mounting solution.
Mounting
Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.

Related Products

P0018　Paraformaldehyde
A2412　DAPI·2HCl
E0197　Evans Blue
A0132　Acridine Orange
A0321　Tris-Base
G0316　Glycerol [for Electrophoresis]
S0111　D-(+)-Sucrose
T3530　Tissue-Clearing Reagent TOMEI [for Plants]

일반 정보

규격치/물성치

규격표

Appearance	Colorless to Almost colorless clear liquid
Functionality test(fluorescence retention)	to pass test
Functionality test(Tissue bleaching)	to pass test

물성치(참고치)

안전 및 규정

GHS

픽토그램
신호 워드	Warning
위험물 및 유해 등록	H315 : Causes skin irritation. H319 : Causes serious eye irritation.
주의 사항	P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention.