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Ready-to-use Solution for Pyrogallol Red-Molybdate Protein Assay
Product
Advantages
• Applicable to determine the amounts of proteins in test samples, because the dye containing pyrogallol red-molybdate complex binds proteins and the absorption maximum of the dye shifts from 480 nm to 600 nm in a linear manner with an increase in the quantity of proteins.
• One-component ready-to-use solution.
• Very little staining of cuvettes which can be washed with water alone after use.
• One-component ready-to-use solution.
• Very little staining of cuvettes which can be washed with water alone after use.
Application
(1) Prepare standard protein solutions with a series of dilutions.
(2) Mix P2575 with unknown protein samples, standard protein solutions and distilled water according to Table 1.
(3) Incubate for 30 minutes at room temperature.
(4) Measure absorbance at 600 nm.
(5) Prepare a standard curve by plotting the absorbance data measured in #4) after subtracting from blank absorbance (distilled water), and calculate the amount of protein in test samples.
Example for use: in a microplate
(1) Prepare four dilution series of standard protein solutions from the concentration at 1000 µg/mL by doubling dilution.
(2) Mix 200 µL of P2575 with 10 µL each of a protein sample at an unknownconcentration, the standard protein solution and distilled water in a 96 microplate.
(3) Incubate for 30 minutes at room temperature, measure absorbance at 600 nm, and prepare a standard curve.
Compatible substance concentrations in protein sample of P2575
Substances at the following concentrations in the sample solutions do not affect the reaction results.
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