Keratanase II, Hydrolytic Enzyme Acting Specifically on Keratan Sulfate

Keratan sulfate is a sulfated polysaccharide classified as a glycosaminoglycan, an essential component of proteoglycans. It is found in tissues such as the cornea of the eye and joints and plays crucial roles in maintaining tissue structure and cell-to-cell signaling. In addition, it has been reported to be involved in muscle and lung diseases, and its function in inflammation and tissue repair is gaining attention. It has also been shown to have properties as a stem cell marker, making it an important subject of research in regenerative medicine and cancer studies.¹⁾
Keratanase II from Bacillus circulans, Recombinant (1) is a recombinant enzyme expressed in Escherichia coli using a gene derived from Bacillus circulans. It specifically acts on keratan sulfate by hydrolyzing the β1-3 linkage between N-acetylglucosamine (GlcNAc) and galactose (Gal). The enzyme requires sulfation at the 6-position of GlcNAc for substrate recognition, but the sulfation of Gal does not affect its activity.²⁾

1 was commercialized under license from Kansai Medical University.

References

• 1) Keratan sulfate, a complex glycosaminoglycan with unique functional capability
  • B. Caterson, J. Melrose, Glycobiology 2018, 28, 182.
• 2) Purification, Characterization, and Molecular Cloning of a Novel Keratan Sulfate Hydrolase, Endo-β-N-acetylglucosaminidase, from Bacillus circulans
  • K. Yamagishi, K. Suzuki, K. Imai, T. Yamagata, J. Biol. Chem. 2003, 278, 25766.

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