Maximum quantity allowed is 999
Please select the quantity
CAS RN: 23491-45-4 | Product Number: H1343
Bisbenzimide H 33258 [for Biochemical Research]
![Thumbnail of Bisbenzimide H 33258 [for Biochemical Research]](/medias/Tci-98-H1343.jpg?context=bWFzdGVyfHJvb3R8MjU5NnxpbWFnZS9qcGVnfGFETXpMMmd4WlM4NU5EVTJOamMxTmpnME16Z3lMMVJqYVMwNU9GOUlNVE0wTXk1cWNHY3w3ZTBiY2FhYzNmYTNhYWQ4OTk3ZWMzOGRhNGRlNjMwNDExM2E5ZmI3NGZiZmNkNWFmZTZmZjcwYjdmZGEzY2Rl "Bisbenzimide H 33258 [for Biochemical Research]")
![Chemical Structure of Bisbenzimide H 33258 [for Biochemical Research]](/medias/Tci-440-H1343.jpg?context=bWFzdGVyfHJvb3R8MTg2NDB8aW1hZ2UvanBlZ3xhR1kwTDJneFpTODVORFUyTmpjMU5qRTRPRFEyTDFSamFTMDBOREJmU0RFek5ETXVhbkJufDFiMDQ3ZTI0MGI4ZjQxMGE1MWE3OTYxNjM5NzEwNjA3OTQxNmFjY2ZkY2EwNjY1NmU5NjFjNGI4YjI3NWQzMDE "Bisbenzimide H 33258 [for Biochemical Research]")
×
![Bisbenzimide H 33258 [for Biochemical Research]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "Bisbenzimide H 33258 [for Biochemical Research]")
Purity: >98.0%(HPLC)
Synonyms:
- 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 25MG |
$55.00
|
≥40 | 4 | Contact Us |
* Please contact our distributors or
TCI
to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Previously this product had been labeled as a hydrate form both in product name and molecular formula. The hydrate form had no exclusive CAS RN and used to share the same CAS RN as the anhydrous form.
To avoid any misleading information and since moisture in product is considered as an impurity, we have decided to remove the term 'Hydrate' from product name and 'xH2O' from the molecular formula for such products.
Please use it at ease since there is no difference in product quality, even if you see the previous indications on a part of label.
| Product Number | H1343 |
Purity / Analysis Method 
|
>98.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__2__5H__2__4N__6O·3HCl = 533.88 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| Packaging and Container
|
25MG-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 23491-45-4 |
| Related CAS RN
|
2138819-37-9 |
| Reaxys Registry Number | 4088183 |
| PubChem Substance ID | 172089156 |
| MDL Number | MFCD00012679 |
Specifications
| Appearance | Light yellow to Brown to Dark green powder to crystal |
| Purity(HPLC) | min. 98.0 area% |
| Water(Vaporization method) | max. 20.0 % |
| DNA staining test | to pass test |
| NMR | confirm to structure |
Properties (reference)
| Melting Point | 280 °C |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
Related Laws:
| RTECS# | SM1140500 |
Transport Information:
| H.S.code* | 2933.59-000 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
Application
Fluorometric Assays of DNA Using Bisbenzimide H 33258
References
- A simple, rapid, and sensitive DNA assay procedure
- Fluorometric assay of DNA in cartilage explants using Hoechst 33258
- Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye
- DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water
- Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG)2 duplex: calorimetric and spectroscopic studies
- Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study
PubMed Literature
Documents
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
Enter TCI Product Number
Enter KISHIDA Product Number
4-5 alphanumeric characters before the hyphen
7 alphanumeric characters
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
• The requested SDS is not available.
Specifications
The requested specifications are not available.
Please Contact Us for more information.
The requested specifications are not available.
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Incorrect Lot Number. Please input only the 7 alphanumeric characters.
• Please check that the lot number you have entered is correct. It is 4-5 alphanumeric characters before the hyphen. In particular, please check O (letter) or 0 (number) and I (letter) or 1 (number).
• If your information is correct and you are still not able to view the requested certificates, Please Contact Us for more information.
• Please check that the lot number you have entered is correct. It is 7 alphanumeric characters. In particular, please check O (letter) or 0 (number) and I (letter) or 1 (number).
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Incorrect Lot Number. Please input only the 7 alphanumeric characters.
The requested analytical chart is not available. Sorry for the inconvenience.
Other Documents
Related Products
![Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/B6236.jpg?context=bWFzdGVyfHJvb3R8MTc2MTF8aW1hZ2UvanBlZ3xhREE1TDJnNU15ODVNVFF3T0RjNE1URTBPRFEyTDBJMk1qTTJMbXB3Wnd8MWEwZWI2OGMxZjUwNmMzYzU2ODU5MjA2MjNkZjk5MTliMGM3MGQ2MmVhNjFjN2I1ZDcxNTcwODcwZTBhNGFmYw "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
| Product # | B6236 |
| CAS RN | 23491-45-4 |
| Purity / Analysis Method: |