Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: H1975
Histone Extraction Kit
×
New
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 1KIT |
$255.00
|
10 | 0 | Contact Us |
* Please contact our distributors or
TCI
to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
- Components : 10x Lysis Buffer, 1x Histone Extraction Buffer, 1x Neutralization Buffer
- Size : for 100 tests
Description
- Histones are essential proteins found in the nucleus of eukaryotic cells. They bind to DNA, packaging it into chromosomes and regulating gene expression.
- This product can be used to isolate histones from cultured mammalian cells and tissues, supporting epigenetic analysis.
Directions for Use
- Dilute 10x Lysis Buffer with purified water to prepare 1x Lysis Buffer.
- Collect cells via centrifugation and remove supernatant. Use a cell scraper to detach adherent cells.
- Resuspend cells in cold 1x Lysis Buffer, adding 1 mL for every 1 x 107 cells.
- Incubate on ice for 10 minutes.
- Centrifuge at 3,000 x g for 5 minutes at 4 °C and remove the supernatant.
- Resuspend the pellet in cold 1x Histone Extraction Buffer. Use 1/10 the amount of buffer used in step 3.
- Incubate on ice for 30 minutes.
- Centrifuge at 12,000 x g for 5 minutes at 4 °C.
- Transfer the supernatant (containing histone) to a new tube.
- Add the same amount of 1x Neutralization Buffer as the 1x Histone Extraction Buffer used in step 6.
Storage
- Store at 4 °C.
| Product Number | H1975 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Documents
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
Enter TCI Product Number
Enter KISHIDA Product Number
4-5 alphanumeric characters before the hyphen
7 alphanumeric characters
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
• The requested SDS is not available.
Specifications
The requested specifications are not available.
Please Contact Us for more information.
The requested specifications are not available.
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Incorrect Lot Number. Please input only the 7 alphanumeric characters.
• Please check that the lot number you have entered is correct. It is 4-5 alphanumeric characters before the hyphen. In particular, please check O (letter) or 0 (number) and I (letter) or 1 (number).
• If your information is correct and you are still not able to view the requested certificates, Please Contact Us for more information.
• Please check that the lot number you have entered is correct. It is 7 alphanumeric characters. In particular, please check O (letter) or 0 (number) and I (letter) or 1 (number).
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Incorrect Lot Number. Please input only the 7 alphanumeric characters.
The requested analytical chart is not available. Sorry for the inconvenience.