Published TCIMAIL newest issue No.199
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CAS RN: | Product Number: B7077
Biotin Quantitation Kit
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Synonyms:
- Biotin Assay Kit
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 1KIT |
€450.00
|
Contact Us | 27 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
- Components : Avidin Buffer 6.25 mL, Biotin Standard (in DMSO) (10mM Biotin in DMSO) 0.1 mL, HABA Buffer 25 mL
- Size : Approx. 34 tests (Cuvette). 170 tests (96-well plate)
Description
- Avidin-biotin binding, the most robust non-covalent protein-ligand interaction in nature, is widely used in biotechnology. When HABA binds to avidin, the resulting complex absorbs light at 500 nm. When a sample containing biotin is added, the HABA is displaced by biotin binding to the avidin. Consequently, the absorbance at 500 nm decreases proportionally to the amount of biotin bound to HABA.
Storage
- Store at 4 ºC. Use within 1 year.
Important Information
- After biotin labeling, remove any unreacted biotinylation reagent from the sample that will be used for biotin quantification using a column or dialysis.
The sample must be dissolved in PBS or TBS for biotin quantification. Avoid buffers containing potassium. If you wish to use a different buffer, compare it with PBS or TBS to ensure there are no issues.
Step-by-Step Protocol
Related Products
- B0463 Biotin
- H0586 4'-Hydroxyazobenzene-2-carboxylic Acid
- B6096 Biotin-LC-LC-NHS (2mg×5)
- A3420 N-(3-Azidopropyl)biotinamide (2mg×5)
- B6294 Biotin-C5-Amine (2mg×5)
| Product Number | B7077 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
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Safety Data Sheet (SDS)
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Specifications
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