2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

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别名：

• 2X SDS-PAGE样品缓冲液 (无2-巯基乙醇) [电泳用]

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SDS | 技术规格 | CoA及其他文档 | 分析图谱

规格	单价	同一天	2-3个工作日	数量
25ML	S$58.50	≥20	2

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Product Information
Volume : 25 mL

Description
This product contains 4% SDS, 20% glycerol, 0.001% bromophenol blue and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 uL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.

Storage
Store at room temperature.

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