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CAS RN: | 产品编码: I1206
Sialidase (Neuraminidase) Agarose
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Product Information
- Preparation : Sialidase is covalently coupled with agarose gel
- Quantity : 0.1 mL gel/0.2 mL (1:1, v/v slurry) suspended in Storage buffer
- Activity : >10 U/mL gel
- Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
- Preservative : 0.02% [w/v] Sodium azide
- Stabilizer : None
Description
- The Sialidase Agarose is an enzyme-immobilized agarose.
This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.
Unit Definition
- One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N- acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.
Assay Conditions
- Reaction conditions: 50 mM Sodium acetate, pH 5.0
The reaction mixture includes the enzyme-immobilized agarose and Neu5Ac-α-4MU in 200 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.
Storage
- Store at 4°C. (Avoid freezing).
Related Products
- G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
- I1205 Sialidase (Neuraminidase)
- Other oligosaccharide-releasing enzymes
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0242 rEGCase II
- R0243 rEGCase II assisted by Activator II
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
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