CAS RN: 89889-52-1 | 產品號碼: B6096
Volume/Quantity : 2 mg x 5
Molecular weight : 567.7
N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6096, Biotin-LC-LC-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 30 mg of antibodies.
This product should be stored at -20 ºC. Do not store at stock solution.
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Directions for Use
Use of a 10 mM Biotin-LC-LC-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-LC-LC-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.
Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM B6096 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol]
A = 20 [uL of 10 mM B6096 solution]
1. Bring Biotin-LC-LC-NHS (Product No. B6096) to room temperature.
2. Dissolve 2 mg of Biotin-LC-LC-NHS (Product No. B6096) in 350uL of dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and prepare a 10 mM solution of Biotin-LC-LC-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-LC-LC-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.
|分子式 / 分子量||C__2__6H__4__1N__5O__7S = 567.7|
|儲存在惰性氣體下||Store under inert gas|
|應避免的情況||Moisture Sensitive,Heat Sensitive|
|Appearance||White to Almost white powder to crystal|
|Functionality test||to pass test|