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CAS RN: 25389-94-0 | 產品號碼: K0047
Kanamycin Monosulfate
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| 產品號碼 | K0047 |
| 純度/分析方法 | >94.0%(N) |
| 分子式 / 分子量 | C__1__8H__3__6N__4O__1__1·H__2SO__4 = 582.58 |
| 外觀與形狀（20°C） | Solid |
| 儲存條件 | Room Temperature (Recommended in a cool and dark place, <15°C) |
| CAS RN | 25389-94-0 |
| 相關CAS RN | 59-01-8&8063-07-8 |
| Reaxys-RN | 3874279 |
| PubChem Substance ID | 87560322 |
| Merck Index(14) | 5281 |
| MDL編號 | MFCD00070253 |
產品規格
| Appearance | White to Almost white powder to crystal |
| Purity(with Total Nitrogen) | min. 94.0 % |
| Specific rotation [a]20/D | +112 to +123 deg(C=1, H2O)(calcd.on dried substance) |
| Drying loss | max. 4.0 % |
性質
| 熔點 | 250 °C(dec.) |
| 比旋光 [α]D | 117.5° (C=1,H2O) |
| 水溶性 | Soluble |
GHS
相關法規
| RTECS # | NZ3225030 |
運輸資料
| HS編碼* | 2941.90-000 |
Application
RNA aptamer research
Reference
- In vitro selection of RNA lectins: using combinatorial chemistry to interpret ribozyme evolution
Application
Kanamycin: A Broad Spectrum Aminoglycoside Antibiotic and a Selective Reagent Genetically Engineered Cells
Kanamycin, an aminoglycoside antibiotic, was first isolated in 1957 from the bacterium Streptomyces kanamyceticus by Umezawa. Kanamycin has broad activity against Gram-negative bacteria and Gram-positive bacteria, including Mycobacterium tuberculosis. Kanamycin interacts with the 30S subunit of prokaryotic ribosomes, and then it inhibits bacterial growth by inhibiting protein synthesis. Kanamycin is used in molecular biology as a selective reagent most commonly to isolate bacteria (e.g., Escherichia coli.) coupled to a gene coding for kanamycin resistance, which is one of the most frequently used selection markers for obtaining transgenic cells.
References
- Design, chemical synthesis, and antibacterial activity of kanamycin and neomycin class aminoglycoside antibiotics
- Kanamycin
- Evaluation of genetic mutations associated with Mycobacterium tuberculosis resistance to amikacin, kanamycin and capreomycin: a systematic review
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
- Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection
- Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant
- Overexpression of an Arabidopsis thaliana ABC transporter confers kanamycin resistance to transgenic plants
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