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CAS RN: 298-93-1 | 產品號碼: M3297
MTT [for Biochemical Research]
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| 產品號碼 | M3297 |
純度/分析方法 
|
>97.0%(HPLC) |
| 分子式 / 分子量 | C__1__8H__1__6BrN__5S = 414.33 |
| 外觀與形狀（20°C） | Solid |
| 儲存條件
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| 儲存在惰性氣體下 | Store under inert gas |
| 應避免的情況 | Light Sensitive,Hygroscopic |
| 包裝和容器
|
1G-Glass Bottle with Plastic Insert （閲覽圖片）, 200MG-Glass Bottle with Plastic Insert （閲覽圖片） |
| CAS RN | 298-93-1 |
| Reaxys-RN | 3825277 |
| PubChem Substance ID | 468592393 |
| SDBS (AIST Spectral DB) | 10829 |
| MDL編號 | MFCD00011964 |
產品規格
| Appearance | Light yellow to Brown powder to crystal |
| Purity(HPLC) | min. 97.0 area% |
| Purity(Nonaqueous Titration) | min. 98.0 % |
| Functionality test | to pass test (by Abs method) |
| Sensitiveness | Colorimetric Test : Turn Red purple with 35ppm D-Glucose |
| NMR | confirm to structure |
性質
| 熔點 | 195 °C(dec.) |
| Maximum Wavelength | 380 nm (H__2O) |
| 水溶性 | Slightly soluble |
| Degree of solubility in water | 10,000 mg/l |
| 溶解性（微溶於） | Ethanol |
GHS
| 圖形表示 |
|
| 信號詞 | Warning |
| 危險性說明 | H315 : Causes skin irritation. H319 : Causes serious eye irritation. H341 : Suspected of causing genetic defects. |
| 防範說明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P202 : Do not handle until all safety precautions have been read and understood. P201 : Obtain special instructions before use. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P405 : Store locked up. |
相關法規
| RTECS # | XF8060000 |
運輸資料
| HS編碼* | 2934.10-000 |
Application
Use of a tetrazolium/formazan assay (MTT assay) for cell growth assays
MTT, a tetrazolium salt, is widely used tool in cell biology for measuring cell proliferation and cytotoxicity, first reported by T. Mosmann in 1983.1) With mammalian cells, fractionation studies indicate that the reduced pyridine nucleotide cofactor (NADH) in active mitochondria, is responsible for reduction of MTT to a blue color formazan product.2) Therefore, the assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells.3)
References
- 1) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
- 2) Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction
- 3) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction (a review)
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產品規格
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其他文件
Related Products
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide
| 產品號碼 | D0801 |
| CAS RN | 298-93-1 |
| Purity #: | >98.0%(T) |