At TCI, we support discovery in life sciences through our knowledge and expertise in organic small molecules. This issue covers our life science topics as follows.

・ Plants Tissue-Clearing Reagent Suitable for Fluorescent Protein Observation: iTOMEI

・ Ready-to-use Solutions for Protein Extraction

・ Lipid Mixture for Easy Preparation of Liposomes: Presome ^®

・ Synthetic Reagent for Unsymmetrical Disulfide Compounds

Plants Tissue-Clearing Reagent Suitable for Fluorescent Protein Observation: iTOMEI

The transparency technique "TOMEI" for plant analysis enables the tissue-clearing of Oryza sativa , Arabidopsis thaliana and many others. On the other hand, improved TOMEI (iTOMEI) is a method that specializes TOMEI for fluorescent protein observation. This method enables simple transparency and allows for clear detection of fluorescent proteins. TCI offers decoloring solution iTOMEI-D [T3940] and mounting solution iTOMEI-M (RI 1.40) [T4003] for iTOMEI method.

Ready-to-use Solutions for Protein Extraction

The first step in protein purification, lysing tissue or cells to extract protein, is crucial for properties and yield of the obtained protein. TCI offers three detergent-based extraction buffers, Nervous Tissue Protein Extraction Buffer [B6279] , RIPA Buffer (Ready-to-use) [R0246] , and E.coli / Yeast Protein Extraction Buffer [Y0021] which are optimized for a wide range of target tissues, from cultured cells to nervous tissue and microorganisms.

Lipid Mixture for Easy Preparation of Liposomes: Presome ^®

Presome ^® ACD-1 [P2807] is the homogeneous mixture of hydrogenated soybean phospholipid, cholesterol and MPEG2000-DSPE (3 : 1 : 1). Liposome liquid solution can be prepared without any organic solvent by simply adding an aqueous solution of the substance to be encapsulated and lightly stirring. You can also see the contributed article on Presome ^® .

Synthetic Reagent for Unsymmetrical Disulfide Compounds

N,N'-Thiodiphthalimide [T4001] can react with a variety of thiols bearing bulky functional groups in mild conditions to form unsymmetrical disulfide bonds via a two-step process. The unsymmetrical disulfide formation is applicable to the cross-linking of biomolecules such as amino acids, sugars, nucleic acids, fluorescent probes etc.