Protein Gel Stain Tangerine (in Dimethyl Sulfoxide) [for Electrophoresis]

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Synonymes

• (E)-4-[4-[2-(9-Ethyl-9H-carbazol-3-yl)vinyl]quinolin-1-ium-1-yl]butane-1-sulfonate (in Dimethyl Sulfoxide)

Documents de produit:

SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

Informations supplémentaires sur le produit

Notes explicatives de cette page

Taille	Prix ​​unitaire	Belgique	Japon *	Quantité	Informations d'expédition
0.25ML	€110.00	Expédié dans un délai de 1 semaine après la commande	10		Simulation d'expédition

*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)

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Product Information

• Volume : 250 µL (5000X concentrated solution in DMSO)

Description

• Protein Gel Stain Tangerine is a fluorescent dye used to stain proteins in gels following SDS-PAGE. Its straightforward staining procedure enables the detection of proteins at concentrations as low as 5-10 ng. This product is fully miscible with water-based solvents, bypassing the use of harsh organic solvents to make it safe for use. When stained in the presence of salts such as 150 mM NaCl, proteins are not fixed in the gel and can be transferred to a blotting membrane. This enables downstream applications such as Western blotting. If protein fixation is to be carried out, this product should first be dissolved in a solution such as 7.5% (v/v) acetic acid. This product should not be used in solutions containing methanol, as methanol removes the SDS coating from proteins, markedly reducing the staining signal. After staining is completed, simply rinse the gel twice with water before imaging. This product exhibits excitation maxima at 290 nm and 490 nm and an emission maximum at 650 nm. Detection can be performed using a UV transillumination system or Imager. Please note that this product is not suitable for staining two-dimensional (2D) gels or isoelectric focusing (IEF) gels.

Storage

• Store at 4 °C. This product is photosensitive and should be protected from light. Use within 6 months.

Step–by–Step Protocol

• See the pdf file in this link.

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