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CAS RN: | Numéro de produit: T3940
Tissue-Clearing Reagent iTOMEI-D [for Plants]
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Pureté:
Synonymes
Documents de produit:
| Taille | Prix unitaire | Belgique | Japon * | Quantité |
Informations d'expédition
|
|---|---|---|---|---|---|
| 5ML |
€29.00
|
1 | 4 |
|
|
| 25ML |
€83.00
|
8 | 26 |
|
*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)
Informations supplémentaires sur le produit:
"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.
Please see a brochure (PDF) of how to use.
Description
This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.
Storage
The product should be stored at 4 °C.
Directions for Use
Required reagents;
- Paraformaldehyde (PFA)
- PBS
- Stains* (option)
- Mounting solution**
==Process outline (All operations are performed at Room temperature)===============
- Tissue fixation
Reagent : 1% PFA in PBS
Time : 1hr - PBS Wash ×3
- Decoloring
Reagent : iTOMEI-D (TCI Product No. T3940)
Time : 24hr - PBS Wash ×3
- (Optional process Staining*, PBS Wash ×3)
- Clearing (RI matching)
Reagent : Mounting solution**
Time : Operation time is determined by the mounting solution. - Mounting
Reagent : Mounting solution**
* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Mounting solution : Please use the suitable mounting solution for objective lens.
Related Products
P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris-Base
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]
| Numéro de produit | T3940 |
| Etat physique (20 ° C) | Liquid |
Condition de stockage 
|
Refrigerated (0-10°C) |
| Condition à éviter | Heat Sensitive |
Spécifications
| Appearance | Colorless to Almost colorless clear liquid |
| Functionality test(fluorescence retention) | to pass test |
| Functionality test(Tissue bleaching) | to pass test |
Propriétés
SGH
| Pictogramme |
|
| Mot de signal | Attention |
| Mentions de danger | H315 : Provoque une irritation cutanée. H319 : Provoque une sévère irritation des yeux. |
| Conseils de prudence | P264 : Se laver la peau soigneusement après manipulation. P280 : Porter des gants de protection/ un équipement de protection des yeux/ du visage. P302 + P352 : EN CAS DE CONTACT AVEC LA PEAU: Laver abondamment à l’eau. P337 + P313 : Si l'irritation oculaire persiste: consulter un médecin. P362 + P364 : Enlever les vêtements contaminés et les laver avant réutilisation. P332 + P313 : En cas d'irritation cutanée: consulter un médecin. |
Lois connexes:
Informations de transport:
| N ° SH (import / export) (TCI-E) | 3822190090 |
Application
The Decoloring Solution for Plant Tissue-Clearing Method iTOMEI
Figure. Cleared Arabidopsis thaliana by “iTOMEI”
(left) Non-treatment (right) iTOMEI treatment
References
- 1) Improved clearing method contributes to deep imaging of plant organs
- 2) Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution
