Maximum quantity allowed is 999
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 1KIT |
₹17,000.00
|
1 | 18 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Product Information
Components : DAB solution, Hydrogen Peroxide solution, 1.5M Tris-HCl buffer
Size : 100 tests (About 500 mL substrate solution)
Description
This kit consists three reagents; 3, 3’-diaminobenzidine (DAB) solution, Hydrogen Peroxide solution and 1.5M Tris-HCl buffer and it is supplied to detect horseradish peroxidase (HRP) activity in Western blotting and so on. When DAB reacts with HRP, a brown precipitate appears. This kit does not include nickel component.
Direction for Use
1) Prepare a blotting membrane covered with HRP-conjugated antibody.
2) Prepare the DAB substrate solution according to the following volume;
- Add 5 drops of DAB solution (about 140 µL)
- Add 2 drops of Hydrogen Peroxide solution (about 80 µL)
- Add 2 drops of 1.5M Tris-HCl buffer (about 80 µL)
- Each reagent is added into 5 mL of deionized water
3) Soak the membrane in the DAB substrate solution and incubate until the membrane is colored.
4) Add deionized water and stop the coloring reaction.
Storage
The product should be stored at 4 °C and used within 6 months.
| Product Number | D5909 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Figure. Dot blot image of substrate solutions prepared by DAB staining kit reacting with HRP (from left to right: 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078 (mU/spot)).
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![Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]](/_ui/responsive/theme-tci/images/missing_product_srp.jpg "Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]")
Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]
| Product # | C4087 |
| CAS RN | |
| Purity / Analysis Method: |
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4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
| Product # | C3384 |
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| Product # | N1113 |
| CAS RN | |
| Purity / Analysis Method: |
