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CAS RN: 1239-45-8 | Product Number: E1363
Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
![Chemical Structure of Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]](/medias/E1363.jpg?context=bWFzdGVyfHJvb3R8NTA4Mzl8aW1hZ2UvanBlZ3xhRGs1TDJnMVlTODRPVEl4TmpZNE1USTBOekF5TDBVeE16WXpMbXB3Wnd8ZWU4ZTc1NmMzMTk0NzA1YjE2MmRmYjMxOTg1ZDg1MmJjOWJmYjhkNDYxYzZlODU1ZTc5YjdjYzAxYWMwNzViOA "Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]")
![Thumbnail of Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]](/medias/E1363.jpg?context=bWFzdGVyfHJvb3R8MTUzNjB8aW1hZ2UvanBlZ3xhR1F6TDJobU5pODRPVE0xTURreE1UUXlOamcyTDBVeE16WXpMbXB3Wnd8MzFhOTM2MGM0MTQ5ZWMyN2FiODZkM2U5YzBmMDBkN2IzNjg2YmMwY2RiZmVmYWU4ZDg5MWFhNjY1YTdjNDQ4NA)
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Purity:
Synonyms:
- 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide (0.5mg/mL in Water)
- Homidium Bromide (0.5mg/mL in Water)
Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 10ML |
₹5,400.00
|
Contact Us | ≥40 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
Volume : 10 mLDescription
This product contain 0.5mg/mL Ethidium Bromide and can use as nucleic acid stain solution or electrophoresis buffer solution. Each drop contains 20 µg of Ethidium Bromide, so you can easily adjust the solution as final concentration. Convenient and safe to use because of dropper bottle.
Storage
The product should be stored at 4 °C, protect from light.
Description for Use
After electrophoresis, dilute an Ethidium Bromide solution to 0.5 μg/mL with water or running buffer and incubate the gel for 15-30 min. Destaining is usually not needed. If you have to decrease background fluorescence, wash the gel in water for 15 minutes. In use of electrophoresis buffer solution, Ethidium Bromide incorporated into nucleic acid and can visualize band immediately by using UV transilluminator.
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E0370 Ethidium BromideL0393 6X Loading Buffer Bromophenol Blue [for Electrophoresis] [for Molecular Biology] (1 mL×3)
L0440 6X Loading Buffer Double BX [for Electrophoresis] [for Molecular Biology] (1 mL×3)
| Product Number | E1363 |
| Molecular Formula / Molecular Weight | C__2__1H__2__0BrN__3 = 394.32 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 1239-45-8 |
| Reaxys Registry Number | 3642536 |
| PubChem Substance ID | 468591733 |
| SDBS (AIST Spectral DB) | 13171 |
| Merck Index (14) | 4731 |
| MDL Number | MFCD00011724 |
Specifications
| Appearance | Light yellow to Yellow to Orange clear liquid |
| DNA staining test | to pass test |
Properties (reference)
GHS
Related Laws:
| RTECS# | SF7950000 |
Transport Information:
Application
DNA Staining Reagents for Agarose Gel Electrohporesis
Figure 1. Agarose gel after staining with ethidium bromide (0.5 mg/mL solution) (taken in-house) 
Figure 2. Agarose gel after staining with 10X Nucleic Acid Stain Blue (taken in-house)
References
- Agarose Gel Electrophoresis for the Separation of DNA Fragments
- Detection of DNA in Agarose Gels
- J. F. Sambrook, D. W. Russell, in Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, New York, 2001, Vol. 1, Chap. 5, 14.
Application
Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
References
-
- Cold Spring Harbor Laboratory Press, New York
- Joseph Sambrook and David W. Russell., Molecular Cloning third edition, A Laboratory Manual, 2001, 5. 14.
- Importance of Binding Affinity for the Activity of a Metallodendritic Chemical Nuclease.
- Efficient removal of ethidium bromide from aqueous solution by using DNA-loaded Fe3O4 nanoparticles
- Cold Spring Harbor Laboratory Press, New York
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Analytical Charts
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Other Documents
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