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CAS RN: | Product Number: T3531
Streptavidin Maleimide Conjugate
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Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 1VIAL |
₹8,400.00
|
Contact Us | 26 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
Volume/Quantity : 0.5 mg
Conjugation : Purified streptavidin was conjugated to Sulfo-SMCC
(TCI : code S0883).
Product Form : Streptavidin Maleimide Conjugate.
Lyophilized in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
Preservative : None
Specific Activity : >10 U/mg , HABA dye-binding method(1)
Description
Streptavidin Maleimide Conjugate is designed to facilitate the conjugation of the Streptavidin to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups.
Protocol
1. Allow the vial of Streptavidin Maleimide Conjugate to warm to room temperature.
2. Reconstitute the Streptavidin Maleimide Conjugate by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
Note: The buffer in which the thiol-containing molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA (1-10 mM) may help to prevent metal catalysed oxidation of thiols.
3. React for 30–60 minutes at 37 °C or 2 hours at room temperature.
4. If desired, desalt, dialyze or purify the conjugate.
Storage
Store at －20 °C to －80 ºC (Preferably at －80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
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| Product Number | T3531 |
| Physical State (20 deg.C) | Solid |
Storage Temperature 
|
Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
| PubChem Substance ID | 354335029 |
Specifications
| Appearance | White to Brown to Dark green powder to crystal |
| Specific Activity | to pass test |
Properties (reference)
GHS
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Application
Streptavidin Maleimide Conjugate
References
- Spectrophotometric determination of avidin and biotin.
- Isolation and characterization of highly purified streptavidin obtained in a two-step purification procedure from Streptomyces avidinii grown in a synthetic medium.
- G. T. Hermanson, in Bioconjugate Techniques, 2nd ed., Academic Press, 2008, pp.905-909.
Documents
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Specifications
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Other Documents
Related Products
Streptavidin from Streptomyces avidinii
| Product # | S0951 |
| CAS RN | 9013-20-1 |
| Purity / Analysis Method: |
Streptavidin DTBTA-Eu3+ Conjugate
| Product # | S0993 |
| CAS RN | |
| Purity / Analysis Method: |