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CAS RN: | Product Number: T3885

Streptavidin R-PE Conjugate


Purity:
Synonyms:
  • SA-R-PE
Documents:
1VIAL
₹8,700
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Note:

Product Information

Volume/Quantity : 0.1 mg
Product Form : Purified streptavidin, R-Phycoerythrin (R-PE) conjugated.
Conjugation : Purified streptavidin was conjugated to R-PE (Ex:496 nm, Em:575 nm)
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Specificity : Biotin

Application

Immunofluorescence (1:200). Flow cytometry (1:1000). FLISA (1:400). Western blotting (1:1000).


Storage

Store at 4 ºC. Do Not Freeze. This product is photosensitive and should be protected from light.


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Product Number T3885
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Light Sensitive,Heat Sensitive
Specifications
Appearance Light red to Red clear liquid to cloudy liquid
Specific Activity to pass test(FLISA)
Properties (reference)
GHS
Related Laws:
Transport Information:
Application
Immunofluorescence Staining

The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL (shown in red
   Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL (shown in green
   Hoechst 33258 [H1343] 1 µg/mL(shown in blue


Application
Flow Cytometry of Stained Cells

The HeLa cells were incubated with Mouse Anti CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line) at 1μg/mL. Then both were incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] at 1 µg/mLas a secondary antibody, followed by staining with Streptavidin R-PE Conjugate [T3885] at 1 µg/mL.

References


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