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CAS RN: | Product Number: T4003
Tissue-Clearing Reagent iTOMEI-M (RI 1.40) [for Plants]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 5ML |
₹2,500.00
|
Contact Us | 12 |
|
| 25ML |
₹5,300.00
|
2 | 28 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product can be used as a mounting agent in iTOMEI. Its refractive index is 1.40, optimized to silicone immersion objectives.
Please see a product brochure (PDF) of how to use.
Description
This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene. The refractive index (RI) of this product is optimized to silicone immersion objectives.
The decoloring solution is also required to complete the iTOMEI method.
Storage
The product should be stored at 4 °C.
Directions for Use
Required reagents
- Paraformaldehyde (PFA)
- PBS
- Stains* (option)
==Process outline (All operations are performed at Room temperature) ===============
- Tissue fixation
Reagent : 1% PFA in PBS
Time : 1hr - PBS Wash ×3
- Decoloring
Reagent : iTOMEI-D (Product No. T3940)
Time : 24hr - PBS Wash ×3
- (Optional process Staining*, PBS Wash ×3)
- Clearing (RI matching)**
Reagent : iTOMEI-M (Product No. T4003)
Time : Operation time is determined by the mounting solution. - Mounting**
Reagent : iTOMEI-M (Product No. T4003)
*The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Please use the suitable mounting solution for objective lens.
(iTOMEI-M is optimized to silicone immersion objectives.)
Related Products
P0018 ParaformaldehydeA2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris(hydroxymethyl)aminomethane
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]
T3940 Tissue-Clearing Reagent iTOMEI-D [for Plants]
| Product Number | T4003 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Functionality test | to pass test (Remaining fluorescence strength) |
Properties (reference)
| Specific Gravity (20/20) | 1.23 |
| Refractive Index | 1.40 |
GHS
Related Laws:
Transport Information:
Application
iTOMEI: Plants Tissue-Clearing Method Suitable for Fluorescent Protein Observation
References
- Improved clearing method contributes to deep imaging of plant organs
- Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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Analytical Charts
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Other Documents
Related Products
Paraformaldehyde
| Product # | P0018 |
| CAS RN | 30525-89-4 |
| Purity / Analysis Method: | >90.0%(T) |
![Tissue-Clearing Reagent iTOMEI-D [for Plants]](/_ui/responsive/theme-tci/images/missing_product_srp.jpg "Tissue-Clearing Reagent iTOMEI-D [for Plants]")
Tissue-Clearing Reagent iTOMEI-D [for Plants]
| Product # | T3940 |
| CAS RN | |
| Purity / Analysis Method: |
![DAPI 2HCl [for Biochemical Research]](/medias/A2412.jpg?context=bWFzdGVyfHJvb3R8MzAzMDV8aW1hZ2UvanBlZ3xhR1kwTDJnMFpDODRPVEk0TXpjek16YzFNREEyTDBFeU5ERXlMbXB3Wnd8Y2YyNTg3YzdkY2E1MzYyNzdmODcyNTNiYmIzN2Y3NTFkMTY1ODY5N2Q0NTBkMjY1ZTBiODFlYjBkZjQwZGVhYw "DAPI 2HCl [for Biochemical Research]")
DAPI 2HCl [for Biochemical Research]
| Product # | A2412 |
| CAS RN | 28718-90-3 |
| Purity / Analysis Method: | >90.0%(HPLC) |