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(1) After electrophoresis, wash the gel with deionized water for 5 minutes three times.
(2) Remove the water used for washing, add C3488 till the gel is soaked, and let the gel stain for 1 hour while shaking gently at room temperature.
(3) Remove the staining solution, destain the gel with deionized water for 1 hour and check it.
(4) If the background is high, destain the gel with deionized water overnight at room temperature.
