Diazirine changes to a high-reactive carbene by absorbing light near 360 nm, which forms a covalent bond to a nearby molecule. The covalent bond is more stable than one formed by nitrene derived from a photoreactive azide.
Phenyldiazirine derivatives 1–3 have a functional group at the para position. For example, after the functional group attaches to a ligand, the diazirine group reacts with the acceptor molecule under photoexcitation. It has been reported in research of the photoaffinity labelings which include the crosslinker with peptides or sugars,2) and the photoaffinity microarrays.3)
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