Ready-to-use Solution for Protein Extraction from Nervous Tissue

　1 is a ready-to-use solution for protein extraction from nervous tissue. By suspending tissue in 1 and then centrifuging, the supernatant containing proteins can be obtained. Extracted protein can be used in downstream applications such as electrophoresis and western blotting.

Application example

[Protein extraction]

1. Wash mouse brain twice with PBS.
2. Weigh samples. For each gram of sample, add 10 mL of 1 and homogenize.
3. Incubate on ice for 10 minutes.
4. Centrifuge the sample at 10000 × g for 10 minutes at 4 °C and collect lysate fraction.

[Protein quantification]: measurement of protein concentration in lysate fraction by pyrogallol red method

[Electrophoresis / Western blotting]: Detection of specific protein in lysate fraction

Synaptophysin (38 kDa) in synaptic vesicle can be extracted from mouse brain efficiently.

References

• 1) Quantification of the Brain Proteome in Alzheimer's Disease Using Multiplexed Mass Spectrometry
• S. Musunuri, M. Wetterhall, M. Ingelsson, L. Lannfelt, K. Artemenko, J. Bergquist, K. Kultima, G. Shevchenko, J. Proteome Res. 2014, 13, 2056.
• 2) Optimized protein extraction from cryopreserved brain tissue samples.
• C. Ericsson, I. Peredo, M. Nistér, Acta Oncol. 2007, 46, 10.

Related Products

P2575

Pyrogallol Red (Ready-to-use solution) [for Protein determination]

D5909

DAB staining kit

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