Hydroxyurea increases transduction by adeno-associated virus (AAV) vectors.1)
Hydroxyurea (or hydroxycarbamide), a hydroxylated molecule of urea [
U0077][
U0073], is classified as an antitumor antimetabolite. Hydroxyurea inhibits deoxynucleotide and DNA synthesis by blocking the cellular enzyme ribonucleotide reductase, which is responsible for producing the deoxynucleotides required for DNA synthesis and repair, producing cell death in S phase (the phase of DNA synthesis). Recently, two effects of hydroxyurea have been reported: hydroxyurea increases the level of fetal hemoglobin, leading to a reduction in the incidence of vaso-occlusive crises in sickle cell anemia, and hydroxyurea inhibits human immunodeficiency virus-type 1 (HIV-1) DNA synthesis. (The product is for research purpose only.)