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Maximum quantity allowed is 999
CAS RN: | Product Number: A2509
Anti-Sialyl Lewis A Monoclonal Antibody (2D3)
Purity:
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Supplemental Product Information:
Product Information
Volume/Quantity: 0.1 mg/vial
Product Form: Purified mouse monoclonal antibody/IgM, κ-chain
Buffer: Phosphate buffered-saline(-)  ((PBS(-))
Preparation: The antibody is purified from ascites fluid. 
Preservative: 0.05% sodium azide
Stabilizer: None
Immunogen: Immunized with human colon cancer cells (Colo201), and screened using sialyl Lewis A-active glycolipids
Specificity: Sialyl Lewis A antigen
Clone Name: 2D3
Applications
Immunohistochemistry, flow-cytometric analysis, ELISA, TLC-immunostaining, Western blotting, cell adhesion inhibition experiments. 
Storage
Store at -20 ºC to -80 ºC (Preferably at -80 ºC). It is not recommended storing at 4 ºC. Avoid repeated freezing and thawing.
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G0453 Goat Anti-Mouse IgM FITC Conjugate
| Product Number | A2509 | 
| Physical State (20 deg.C) | Liquid | 
Storage Temperature  
                                     | 
                                    Frozen (-80°C) | 
| Condition to Avoid | Heat Sensitive | 
| Appearance | Colorless to Almost colorless clear liquid | 
| Titer(ELISA) | to pass test | 
| HS Number | 3504.00.5000 | 
References
- Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A
 - Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to cancer-associated ganglioside antigens having various sialic acid linkages				     				 
- K. Zenita, K. Hirashima, K. Shigeta, N. Hiraiwa, A. Takada, K. Hashimoto, E. Fujimoto, K. Yago, R. Kannagi, J. Immunol. 1990, 144, 4442-4451.
 
 - Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium				     				 
- A. Takada, K. Ohmori, T. Yoneda, K. Tsuyuoka, A. Hasegawa, M. Kiso, R. Kannagi, Cancer Res. 1993, 53, 354-361.
 
 - Importance of E-selectin (ELAM-1) and sialyl Lewisa in the adhesion of pancreatic carcinoma cells to activated endothelium
 - Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates
 - Clinical application of functional glycoproteomics - dissection of glycotopes carried by soluble CD44 variantsin sera of patients with cancers
 - Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors, siglec-7 and -9				     				 
- K. Miyazaki, K. Sakuma, Y. I. Kawamura, M. Izawa, K. Ohmori, M. Mitsuki, T. Yamaji, Y. Hashimoto, A. Suzuki, Y. Saito, T. Dohi, R. Kannagi, J. Immunol. 2012, 188, 4690-4700.
 
 - Peritoneal colonization by human pancreatic cancer cells is inhibited by antisense FUT3 sequence
 - Detection of ligands for selectins in the oligosaccharide fraction of human milk
 
Documents
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Specifications
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