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CAS RN: | Product Number: D6539

DiO Solution [for Cell Membrane Staining]

Chemical Structure of DiO Solution [for Cell Membrane Staining]
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× DiO Solution [for Cell Membrane Staining]

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Supplemental Product Information

Explanatory notes for this page

1ML
$180.00
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Supplemental Product Information:

Product Information

  • Concentration : 1 mM
  • Excitation / Emission : 491 nm / 509 nm

Description

  • DiO is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiO which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

  • Store at room temperature (below 15 °C is recommended) and protect from light.

Directions for Use: Staining live cells

    1. Dilute DiO solution to 5 μM with culture medium.
    2. Remove medium from cells and replace with 5 μM DiO-containing medium.
    3. Incubate cells at 37 °C for 10 minutes.
    4. Remove medium and wash cells 3 times with PBS.
    5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

    1. Remove culture medium and wash cells twice with PBS.
    2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
    3. Dilute DiO solution to 5 μM with PBS.
    4. Remove 4% paraformaldehyde and add 5 μM DiO-containing PBS to cells.
    5. Incubate cells at 37 °C for 10 minutes.
    6. Remove medium and wash cells 3 times with PBS.
    7. Observe stained cell membranes via a fluorescence microscope.

Related Products

  • D6202 DiI Solution [for Cell Membrane Staining]
  • D6263 DiD Solution [for Cell Membrane Staining]
  • D6540 DiR Solution [for Cell Membrane Staining]
  • P3316 PKH 26 Solution [for Cell Membrane Staining]

Product Number D6539
Physical State (20 deg.C) Liquid
Storage Temperature Room Temperature (Recommended in a cool and dark place, <15°C)
Store Under Inert Gas Store under inert gas
Condition to Avoid Air Sensitive
Specifications
Appearance Colorless to Yellow clear liquid
Functionality test to pass test
Properties (reference)
GHS
Pictogram Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H319 : Causes serious eye irritation.
H360 : May damage fertility or the unborn child.
H372 : Causes damage to organs through prolonged or repeated exposure.
H373 : May cause damage to organs through prolonged or repeated exposure.
H350 : May cause cancer.
H402 : Harmful to aquatic life.
H225 : Highly flammable liquid and vapor.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P273 : Avoid release to the environment.
P270 : Do not eat, drink or smoke when using this product.
P202 : Do not handle until all safety precautions have been read and understood.
P240 : Ground/bond container and receiving equipment.
P260 : Do not breathe mist or vapours.
P210 : Keep away from heat/sparks/open flames/hot surfaces. No smoking.
P233 : Keep container tightly closed.
P201 : Obtain special instructions before use.
P243 : Take precautionary measures against static discharge.
P241 : Use explosion-proof electrical/ ventilating/ lighting/ equipment.
P242 : Use only non-sparking tools.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P308 + P313 : IF exposed or concerned: Get medical advice/ attention.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P303 + P361 + P353 : IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.
P403 + P235 : Store in a well-ventilated place. Keep cool.
P405 : Store locked up.
Related Laws:
Transport Information:
UN Number (DOT-AIR) UN1170
Class (DOT-AIR) 3
Packing Group (TCI-A) II
HS Number 3822.19.0080
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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