Clearing Reagent iCBiofilm-H1 [for Biofilm]

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Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity
8ML	$81.00	Contact Us	Contact Us	23

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Product Information

Description

Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-H1 has been developed to allow for the observation of biofilm structure at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-H1 solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques.

*This product was developed by Associate Prof. Sugimoto of the Jikei University School of Medicine.

Storage

Store at room temperature. Use within 6 months.

Directions for Use

1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
4. Stain using appropriate fluorescence reagents.
5. Remove staining reagents and add 500 µL - 1 mL of Clearing Reagent iCBiofilm-H1 (Product No.T4031).
6. Observe biofilm using optical microscopy.

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