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CAS RN: | Product Number: D6131
DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]
![DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research] Chemical Structure of DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]](/medias/Tci-440-D6131.jpg?context=bWFzdGVyfHJvb3R8MTgzNjd8aW1hZ2UvanBlZ3xhREF5TDJneU15ODVOREUxTkRBM05UazVOalEyTDFSamFTMDBOREJmUkRZeE16RXVhbkJufGMyYzcxODFiNWYyNTExMWNkYjM4NTlmZmFkMmZjMWIxMDQ1NGNmN2Q1NzhkYjE4M2ZiMGQ5ZWIzYjczNmI5NDE)
![DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research] Chemical Structure of DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]](/medias/Tci-440-D6131-3T3-L1-adipocytes-stained-by-DBC30.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8MjA1NjYyfGltYWdlL2pwZWd8YUdVNEwyZzJZaTg1TlRFME1UTXpOREl5TVRFd0wxUmphUzAwTkRCZlJEWXhNekV0TTFRekxVd3hMV0ZrYVhCdlkzbDBaWE10YzNSaGFXNWxaQzFpZVMxRVFrTXpNQzVxY0djfGY3MTNiZmE2YjUyMGJkYzg5MzAwYWE0MzYzN2IxMjZlZmMzZmQ3NzY3NDRjMDUxOTgxY2VjMWQ1YzZhOThiMTE)
Purity:
Synonyms:
- 7-(Dibutylamino)-3-(1-methyl-1H-benzimidazol-2-yl)-2H-chromen-2-one (2mg/mL in Dimethyl Sulfoxide)
Product Documents:
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Supplemental Product Information:
Product Information
- Product Form : DMSO solution
- Excitation / Emission : 403 nm / 468 nm
Description
- DBC30 is a small compound that emits strong fluorescence in hydrophobic environment. It can dye clearly not only cellular lipid droplets but also tissue lipid droplets. It can be used in combination with multiple fluorescent dyes.
This product was developed by Dr. Toshitada Yoshihara.
Direction for Use
- Dilute DBC30 solution to 40 μg/mL (100 μM) with DMSO, then dilute to 40 ng/mL (100 nM) with medium such as DMEM.
- Remove culture medium and wash cells twice with PBS.
- Add 100 nM DBC30-containing medium to cells.
- Incubate cells at 37 °C for 30 minutes.
- Remove medium and wash cells twice with PBS.
- Observe stained lipid droplets.
Directions for Use: Normal or fatty liver mouse imaging
- Dilute DBC30 solution to 200 μg/mL (500 μM) with saline containing 10% BSA.
- Inject the 100 µL of 500 µM DBC30 stock solution into the tail vein of an eight-week-old mouse under anesthesia 15 minutes before the fluorescence imaging experiment.
- Expose the liver of the mouse and observe it.
Storage
- Store at -20 °C and protect from light. Avoid repeated freezing and thawing.
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Product Number | D6131 |
Molecular Formula / Molecular Weight | C__2__5H__2__9N__3O__2 = 403.53 |
Physical State (20 deg.C) | Liquid |
Storage Temperature ![]() |
Frozen (-20°C) |
Store Under Inert Gas | Store under inert gas |
Condition to Avoid | Light Sensitive,Air Sensitive,Heat Sensitive |
Reaxys Registry Number | 44141154 |
Specifications
Appearance | Light yellow to Yellow to Green clear liquid |
Lambda max. | 400.0 to 430.0 nm |
Maximal emission wavelengths | 470.0 to 500.0 nm |
Cell staining test | to pass test |
Properties (reference)
GHS
Signal Word | Warning |
Hazard Statements | H227 : Combustible liquid. |
Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P403 : Store in a well-ventilated place. |
Related Laws:
Transport Information:
H.S.code* | 2934.99-000 |
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