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CAS RN: 124522-09-4 | Product Number: A5553

3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one [for HPLC Labeling]


Purity: >98.0%(T)
Synonyms:
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Product Number A5553
Purity / Analysis Method >98.0%(T)
Molecular Formula / Molecular Weight C__1__0H__8BrNO__3 = 270.08  
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 124522-09-4
PubChem Substance ID 87563171
MDL Number

MFCD00143099

Specifications
Appearance Light yellow to Brown powder to crystal
Purity(HPLC) min. 97.0 area%
Purity(Argentometric Titration) min. 98.0 %
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
Transport Information:
HS Number 2934.99.4400
Application
HPLC Labeling Reagent for Carboxylic Acids

The compound 1 is an HPLC fluorescence labeling reagent, which has a bromomethyl group, can easily react with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 355 nm and 430 nm, respectively.

Application examples:
[Fatty acids] 1)
A solution of the labeling reagent 1 (0.1 mL, 1.0 mM acetonitrile solution) is added to a solution of a fatty acid (0.5 mL, 0.2~10 nmol in acetonitrile). To this solution, a saturated K2CO3 / acetonitrile solution (0.5 mL) containing 18-crown 6-ether (5.7 mM) is added, and incubate at 40 °C for 30 min. After cooling to room temperature, use it as the HPLC sample solution.

References


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