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CAS RN: 139332-64-2 | Product Number: A5555

DBD-PZ [=4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole] [for HPLC Labeling]

Purity: >98.0%(HPLC)
  • 4-(N,N-Dimethylaminosulfonyl)-7-piperazinobenzofurazan
  • 4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole
2   1   ≥20 

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Product Number A5555
Purity / Analysis Method >98.0%(HPLC)
Molecular Formula / Molecular Weight C__1__2H__1__7N__5O__3S = 311.36  
Physical State (20 deg.C) Solid
CAS RN 139332-64-2
Reaxys Registry Number 19710060
PubChem Substance ID 87563173
MDL Number


Appearance Light yellow to Yellow to Orange powder to crystal
Purity(HPLC) min. 98.0 area%
Melting point 121.0 to 125.0 °C
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid
Properties (reference)
Melting Point 123 °C
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
Transport Information:
HS Number 2935.90.9500
HPLC Labeling Reagent for Carboxylic Acids

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a piperazino group, easily reacts with a carboxyl group at room temperature to form the corresponding amide in the presence of a condensation reagent. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 440 nm and 569 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. A highly sensitive analysis can be done by peroxyoxalate chemiluminescence detection1).

Application examples:
[Fatty acids] 2)
0.2 mL of 140 mM DEPC or 70 mM 2,2’-dipyridyl disulfide-Ph3P / DMF solution containing a fatty acid (10 µM) is added to 0.2 mL of the labeling reagent 1 / DMF or acetonitrile solution (10 mM). Incubate at room temperature for 6 h, then use it as an HPLC sample.
For example, the detection limit (S/N = 3) for saturated fatty acids (from C13 to C24) is from 3.2 to 4.7 fmol.


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