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CAS RN: 29270-56-2 | Product Number: A5593

NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]

Purity: >99.0%(HPLC)
  • 4-Fluoro-7-nitrobenzofurazan
  • 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
12   8   ≥20 

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Product Number A5593
Purity / Analysis Method >99.0%(HPLC)
Molecular Formula / Molecular Weight C__6H__2FN__3O__3 = 183.10  
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Air Sensitive,Heat Sensitive
CAS RN 29270-56-2
Reaxys Registry Number 1077571
PubChem Substance ID 87563200
SDBS (AIST Spectral DB) 50330
MDL Number


Appearance White to Light yellow to Green powder to crystal
Purity(HPLC) min. 99.0 area%
Melting point 53.0 to 57.0 °C
Effect test of HPLC derivatization Effective as labeling agent for L-Isoleucine
Properties (reference)
Melting Point 55 °C
Solubility (soluble in) Chloroform
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
Transport Information:
HS Number 2934.99.4400
HPLC Labeling Reagent for Amines and Thiols

The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with amino or mercapto groups to form the corresponding derivatives. 1 itself does not fluoresce, and its ethanol solution is relatively stable for a week in a refrigerator. The derivatives can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 530 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. Thus, further highly sensitive detection can be done by using laser induced fluorescence detector. When the reagent is hydrolyzed (NBD-OH), its fluorescence can be erased under an acidic condition. Therefore, this hydrolyzed reagent can be used as a post column reaction reagent5,7).

Application example:
[Amino acids] 2,3)
To 10 µL of 50 µM amino acid standard solution, add 10 µL of 0.1 M boric acid buffer solution (pH 8.0) and 20 µL of 50 mM labeling reagent 1 in ethanol solution, and incubate the mixture at 60 °C for 1 min. Immediately cool it with ice bath, and add 460 µL of 5 mM HCl to the reactant solution. Use 10 µL of the solution as an HPLC sample.


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