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CAS RN: | Product Number: B6105

6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]


Purity:
Synonyms:
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10ML
$62.00
1   Contact Us ≥80 

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Note:

Product Information

Volume : 10 mL

Description

This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25 - 30 °C if precipitation occurs during storage.


Description for Use

Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95 - 100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (TCI Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (TCI Product No. D3647), etc.


Storage

Store at room temperature.


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Product Number B6105
Physical State (20 deg.C) Liquid
Storage Temperature Room Temperature (Recommended in a cool and dark place, <15°C)
Specifications
Appearance Green to Dark green to Dark blue clear liquid to cloudy liquid
Functionality test to pass test(Electrophoresis)
Properties (reference)
Specific Gravity (20/20) 1.16
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
Transport Information:
HS Number 3822.19.0080
Application
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

References


Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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