text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Please select the quantity

CAS RN: 25952-53-8 | Product Number: D1601

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride [Coupling Agent for Peptides Synthesis]

Purity: >98.0%(T)
  • 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride
  • EDC·HCl
  • EDAC·HCl
  • EDCI·HCl
≥40  31   ≥40 
≥40  29   ≥40 
7   6   ≥40 
25   38   ≥40 

* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.

Product Number D1601
Purity / Analysis Method >98.0%(T)
Molecular Formula / Molecular Weight C__8H__1__7N__3·HCl = 191.70 
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Moisture Sensitive,Heat Sensitive
CAS RN 25952-53-8
Reaxys Registry Number 5764110
PubChem Substance ID 87568083
SDBS (AIST Spectral DB) 21309
MDL Number


Appearance White powder to crystal
Purity(with Total Nitrogen) min. 97.0 %
Purity(Argentometric Titration) min. 98.0 %
Properties (reference)
Melting Point 115 °C
Solubility in water Completely soluble
Solubility (soluble in) Dimethylformamide,Alcohol
Pictogram Pictogram
Signal Word Warning
Hazard Statements H302 : Harmful if swallowed.
H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P270 : Do not eat, drink or smoke when using this product.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
P301 + P312 + P330 : IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth.
Related Laws:
RTECS# FF2200000
Transport Information:
HS Number 2925.19.4200
TCI Practical Example: Condensation

Used Chemicals


1-(2-methoxyphenyl)piperazine (500 mg, 2.60 mmol) was dissolved in DMF (15 mL). To this solution was added N-benzyloxycarbonyl-L-phenylalanine (770 mg, 2.60 mmol) and HOBt·H2O (414 mg, 2.71 mmol). The reaction mixture was cooled to 0 °C, then EDC·HCl (515 mg, 2.71 mmol) was added. Reaction was allowed to warm to room temperature, then stirred for 20 h at room temperature. Complete of the reaction was confirmed by TLC. Reaction was quenched by adding H2O (20 mL). Reaction mixture was extracted by EtOAc (30 mL). Organic phase was washed with H2O (20 mL, twice) and brine (20 mL), dried over Na2SO4, concentrated under reduced pressure to afford the crude product (1.2 g) as pale yellow oil. The crude product was purified by column chromatography on silica gel (hexane : ethyl acetate = 40 : 60) to afford the product 1 as a white powder (1.10 g, 2.32 mmol, 90%).

Experimenter's Comments

The reaction mixture was monitored by TLC (hexane / ethyl acetate = 4 : 6, Rf = 0.40)

Analytical Data(Compound 1)

1H NMR (400 MHz, CDCl3); δ 7.39-7.16 (m, 10H), 6.99 (m, 1H), 6.91-6.84 (m, 2H), 6.78 (m, 1H), 5.73 (d, J = 8,2 Hz, 1H), 5.12-5.01 (m, 2H), 4.91 (dd, J= 7.7, 7.3 Hz, 1H), 3.82 (s, 3H), 3.71-3.63 (m, 2H), 3.51 (m, 1H), 3.31 (m, 1H), 3.07-2.78 (m, 5H), 2.51 (m, 1H).

Formation of Peptide Bonds Using EDC·HCl /HOBt

Typical Procedure: To a solution of Boc-ΔTyr(Cl2Bzl)-OH (307 mg, 0.7 mmol), glycinamide hydrochloride (77.4 mg, 0.7 mmol) and Et3N (0.1 mL, 0.7 mmol) in DMF (7 mL) is added EDC·HCl (148 mg. 0.77 mmol) and HOBt (129 mg, 0.84 mmol) at -10°C. The reaction mixture is stirred for 2 h at 0°C. and overnight at room temperature. After the solution is evaporated, the residual is dissolved in EtOAc. The solution is washed with 4% NaHCO3, 10% citric acid and water. The solution is dried and evaporated. The purification is carried out by silica gel column chromatography to give Boc-ΔTyr(Cl2Bzl)-Gly-NH2 (317 mg, 85% yield).
(Δ means dehydro-form.)


  • Dehydro‐enkephalins

  • Application
    Water-soluble Condensation Reagent

    Typical Procedure: To a 50 mL round bottom flask equipped with a magnetic stir bar and septum is added 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 2.54 g (13.3 mmol), 4-dimethylaminopyridine 1.62 g (13.3 mmol), carboxylic acid 1.02 g (2.46 mmol), alcohol 0.96 g (3.98 mmol) and CHCl3 (15 mL), and the reaction mixture is stirred at room temperature for 2.5 days. Acetic acid (0.5 mL) is added and the reaction mixture is stirred for 10 min to convert excess alcohol to a more easily separable acetate. The mixture is washed with saturated aqueous NH4Cl solution, diluted with Et2O, and the layers are dried, filtered and concentrated under reduced pressure. The crude material is chromatographed by silica gel column chromatography (hexane:ethyl acetate = 9:1) to give the desired product 1.25 g (1.96 mmol, 80%).


Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.

The requested SDS is not available.

Please Contact Us for more information.

C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number is entered
Analytical Charts
Please enter Lot Number Incorrect Lot Number is entered

The requested analytical chart is not available. Sorry for the inconvenience.

Other Documents

Session Status
Your session will timeout in 10 minutes. You will be redirected to the HOME page after session timeout. Please click the button to continue session from the same page. minute. You will be redirected to the HOME page after session timeout. Please click the button to continue session from the same page.

Your session has timed out. You will be redirected to the HOME page.