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CAS RN: 298-93-1 | Product Number: M3297

MTT [for Biochemical Research]

Purity: >97.0%(HPLC)
  • 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide
  • Thiazolyl Blue Tetrazolium Bromide
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Product Number M3297
Purity / Analysis Method >97.0%(HPLC)
Molecular Formula / Molecular Weight C__1__8H__1__6BrN__5S = 414.33  
Physical State (20 deg.C) Solid
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Hygroscopic
CAS RN 298-93-1
Reaxys Registry Number 3825277
SDBS (AIST Spectral DB) 10829
MDL Number


Appearance Light yellow to Brown powder to crystal
Purity(HPLC) min. 97.0 area%
Purity(Nonaqueous Titration) min. 98.0 %
Functionality test to pass test (by Abs method)
Sensitiveness Colorimetric Test : Turn Red purple with 35ppm D-Glucose 
Properties (reference)
Melting Point 195 °C(dec.)
Maximum Absorption Wavelength 380(H2O) nm
Solubility in water Slightly soluble
Degree of solubility in water 10,000 mg/l  
Solubility (slightly sol. in) Ethanol
Pictogram Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
H341 : Suspected of causing genetic defects.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P202 : Do not handle until all safety precautions have been read and understood.
P201 : Obtain special instructions before use.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P308 + P313 : IF exposed or concerned: Get medical advice/ attention.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
P405 : Store locked up.
Related Laws:
RTECS# XF8060000
Transport Information:
HS Number 2934.10.9000
Use of a tetrazolium/formazan assay (MTT assay) for cell growth assays

MTT, a tetrazolium salt, is widely used tool in cell biology for measuring cell proliferation and cytotoxicity, first reported by T. Mosmann in 1983.1) With mammalian cells, fractionation studies indicate that the reduced pyridine nucleotide cofactor (NADH) in active mitochondria, is responsible for reduction of MTT to a blue color formazan product.2) Therefore, the assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells.3)


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