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CAS RN: | Product Number: M3527

Mitochondrial Isolation Kit


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1KIT
$351.00
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Product Information

  • Components : Mitochondrial Isolation Reagent A, Mitochondrial Isolation Reagent B, Mitochondrial Isolation Reagent C
  • Size : 30 tests

Description

  • This product can be used to quickly and easily isolate mitochondria from cultured mammalian cells and tissue. Isolated mitochondria can be used in downstream analyses.

Directions for Use

    1. Collect cells via centrifugation and carefully remove supernatant. Use a cell scraper for detachment of adherent cells.
    2. Resuspend cells in Mitochondrial Isolation Reagent A and vortex for 5 seconds. Add 400 μL for every 1 x 107 cells.
    3. Incubate on ice for 2 minutes.
    4. Add Mitochondrial Isolation Reagent B to cell suspension and vortex for 5 seconds. Use 5 μL for every 400 μL of suspension from step 2.
    5. Incubate on ice for 5 minutes, vortexing once per minute.
    6. Add Mitochondrial Isolation Reagent C and invert several times (do not vortex). Use a volume equivalent to that used of Reagent A in step 2.
    7. Centrifuge at 700 x g for 10 minutes at 4°C.
    8. Transfer the supernatant to a new tube and centrifuge at 12,000 x g for 15 minutes at 4°C.
    9. Transfer the supernatant (cytoplasmic fraction) to a new tube and add 300 μL of Mitochondrial Isolation Reagent C to the pellet (mitochondria).
    10. Centrifuge at 12,000 x g for 5 minutes at 4°C and remove supernatant.
    11. Mitochondria (pellet) are now ready to be used in further experiments.

Storage

  • Store at 4 °C.

Product Number M3527
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Heat Sensitive
Specifications
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 3822.19.0080

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