rEGCase I

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Purity:

Synonyms:

• Recombinant Endoglycoceramidase I
• Recombinant - Rhodococcus equi EGCase I expressed in Rhodococcus erythropolis

Documents:

SDS | Specifications | C of A & Other Certificates | Analytical Charts

Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity
1VIAL	$275.00	Contact Us	Contact Us	33

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Product Information

• Quantity : 300 mU per vial (6 mU/μL)
• Product Form : Purified recombinant EGCase I, Liquid
• Storage buffer : 20 mM Sodium phosphate, 50 mM NaCl buffer, pH 7.0
• Optimum pH : pH 5.5
• Preservative : None
• Stabilizer : None

Description

• Endoglycoceramidase I (EGCase I) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under conditions requiring a detergent or a cofactor Activator II. The EGCase I gene is derived from Rhodococcus equi and its recombinant protein is expressed in Rhodococcus erythropolis.

Unit Definition

• One unit (U) of EGCase I is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH 5.5.

Assay Conditions

• Reaction conditions: 50 mM sodium acetate containing 0.4% Triton X-100, pH 5.5
  The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL. The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference).
• [Reference]
  Determination of Glucose by a Modification of Somogyi-Nelson Method
  C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.

Storage

• Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.

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• [Endo-type N-glycanase (ENGase)]
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• [Endo-type O-glycanase]
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• [Endo-type glycosaminoglycanase]
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