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Biotinylation Reagents with Diazirine and Azide for Photoproximity Labeling

To understand basic biology and develop new treatments for diseases, it is important to identify biological interaction networks, known as interactomes. Proximity labelling, specifically photoproximity labeling, is one of the most effective methods for achieving this.1) By irradiating light, short-lived reactive active species probes are generated. These probes diffuse through the solution and covalently bind to nearby biomolecules, thus imparting a tag.2,3,4,5,6) For instance, it has been reported that antibodies covalently linked to photoreactive catalysts can label the microenvironment surrounding antibody-bound proteins through activation of probes. This technique does not necessitate the use of genetic modification technology.2)

The labelling radius of the probes used in proximity labeling affects the scale and resolution of the interactome being mapped. We offer commercial products with different labeling radii for proximity labelling.

A diazirine group, which has a singlet carbene as the reactive active species, is extremely reactive to water. Therefore, the labeling time is only a few minutes and the labeling radius is around 50 nm.6) Arylazide groups, in contrast, generate triplet nitrenes as reactive species and exhibit slow reactivity towards water. As a result, the labeling process takes approximately 10 minutes, and a labeling radius of 50-100 nm can be achieved.6,7,8,9)

Additionally, we offer difunctional photoreactive labeling agents that can be photo-crosslinked at the diazirine moiety and have an alkyne moiety as building blocks.

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