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Rapid Transparency Reagent for Plants: TOMEI
The transparency technique "TOMEI" for plant analysis was developed by Prof. Matsunaga et al. at Tokyo University of Science. This method enables the clearing of Oryza sativa, Arabidopsis thaliana and many others in just a few hours. Using this technique, deeper internal structure can be clearly observed. TCI offers TOMEI reagent for this transparency techique.
Product
*This item is unavailable except in Japan, U.S. and Europe.Advantages
- Simple and rapid clearing of plant can be performed.
- Deeper part can be observed by using confocal microscope.
Applications
The transparency technique "TOMEI-I" reduces the intrinsic fluorescence of chlorophyll, as well as reduced fluorescent protein, and thus observation benefits from the use of fluorescent dyes.
Alternatively, transparency technique "TOMEI-II" doesn't significantly reduce intrinsic fluorescence due to its mild conditions, but the transparency is adequate to detect fluorescent proteins.
References
- Three-Dimensional Imaging of Plant Organs Using a Simple and Rapid Transparency Technique
- The coordination of ploidy and cell size differs between cell layers in leaves