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We know that when researching exosomes, the extraction of high-purity, intact exosomes from your samples is of utmost importance. That's why TCI offers ready-to-use solutions optimized for exosome isolation from serum and cell culture supernatant.
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Example of Exosome Isolation from Human Serum Using E1553
- Centrifuge human serum sample at 3,000 x g for 15 minutes at 4 °C.
- Transfer the supernatant containing clarified serum to a new tube.
- Add Exosome Isolation Reagent [for Serum] [E1553] to the serum and mix via pipetting until homogeneous. Use 20 μL of E1553 per 100 μL serum.
- Incubate for 30 minutes at 4 °C.
- Centrifuge at 10,000 x g for 10 minutes at 4 °C.
- Remove the supernatant carefully without disturbing the pellet (containing exosomes).
- Resuspend pellet in 1x PBS. Use 25-50 μL of PBS per 100 μL of starting serum.
Example of Exosome Isolation from Cell Culture Supernatant Using E1601
- Collect culture supernatant of MCF7 cells and centrifuge at 3,000 x g for 15 minutes at 4 °C.
- Transfer the supernatant to a new tube.
- Add Exosome Isolation Reagent [for Cell Culture Media] [E1601] to the supernatant and mix via pipetting until homogeneous. Use 500 μL of E1601 per 1 mL supernatant.
- Incubate at 4 °C overnight.
- Centrifuge at 10,000 x g for 1 hour at 4 °C.
- Remove the supernatant carefully (pellet is often invisible).
- Resuspend pellet in 1x PBS. Use 25-50 μL of PBS per 1 mL of starting media.