TCI utilise des cookies pour personnaliser et améliorer votre expérience utilisateur. En poursuivant votre navigation sur notre site, vous acceptez l'utilisation de cookies. Vous pouvez modifier ou mettre à jour vosparamètres de cookies à tout moment.
Cypridina luciferin analogs achieve chemiluminescence through reaction with superoxide (O2–) or singlet oxygen (1O2). Using this characteristic, CLA and MCLA have been used in the research of the functions of leukocytes. The maximum emission wavelengths of CLA and MCLA are 380nm and 465nm, respectively. FCLA developed by Goto et al. is characterized by emitting light at a longer wavelength (532nm). Furthermore, Red-CLA developed by Teranishi emits at an even longer wavelength. Red-CLA exhibits high emission intensity by reaction with superoxide, and can be used for efficient analysis of superoxide at the longest wavelength (610nm).
Firefly luciferin reacts with ATP in the presence of luciferase and magnesium ion to provide oxyluciferin via luciferyl-adenylic acid. The light with a wavelength of 562nm is emitted when activated molecules return to the ground state by decomposition of oxyluciferin. Using this characteristic, firefly luciferin is used in the trace detection of ATP and the activity measurement of nucleotide phosphatase etc.
Luminol reacts with hydrogen peroxide in the presence of metals such as iron or its complexes to emit strong blue light with a wavelength of 460nm. This reaction is called the luminol test and is applied to the identification of blood stains in forensic science. Luminol is also used for trace detection of hydrogen peroxide and metals which catalyze this reaction.
On the other hand, oxalates are oxidized by hydrogen peroxide etc. to produce 1,2-dioxetanediones. When these substances are decomposed, they transfer energy to coexisting fluorescent substances which are elevated to the excited state. These excited fluorescent substances emit light during relaxation to the ground state. Thus, the emission wavelength can be changed by choosing coexisting fluorescent substances. This technique is applied to HPLC detection systems.
- (a) T. Kobayashi, K. Saga, S. Shimizu, T. Goto, Agric. Biol. Chem. 1981, 45, 1403.
- (b) M. Nakano, K. Sugioka, Y. Ushijima, T. Goto, Anal. Biochem. 1986, 159, 363.
- (a) A. Nishida, H. Kimura, M. Nakano, T. Goto, Clin. Chim. Acta 1989, 179, 177.
- (b) A. Takahashi, H. Totsune-Nakano, M. Nakano, S. Mashiko, N. Suzuki, C. Ohma, H. Inaba, FEBS Lett. 1989, 246, 117.
- (c) A. Takahashi, M. Nakano, S. Mashiko, H. Inaba, FEBS Lett. 1990, 261, 369.
- (d) T. Sakurai, K. Sugioka, M. Nakano, Biochim. Biophys. Acta - Lipids and Lipid Metabolism 1990, 1043, 27.
- (e) S. Koga, M. Nakano, K. Uehara, Arch. Biochem. Biophys. 1991, 289, 223.
- 3)CLA, MCLA
- (a) M. Nakano, Tanpakushitu, Kakusan, Koso 1988, 33, 2699.
- (b) H. Sawada, M. Nakayama, Kagaku to Kogyo (Tokyo) 1988, 41, 1163.
- (c) H. Sawada, M. Nakayama, Yukagaku 1989, 38, 103.
- (d) M. Nakano, H. Kimura, Rinsho Kensa 1989, 33, 256.
- (e) K. Imai, in Bioluminescence and Chemiluminescence, Hirokawa Publishing, 1989.
- (f) H. Sawada, K. Masuyama, M. Nakayama, Yukagaku 1990, 39, 47.
- (g) M. Nakano, Methods Enzymol. 1990, 186, 227.
- (h) M. Nakano, Methods Enzymol. 1990, 186, 585.
- 4)CLA, MCLA, FCLA
- (a) N. Suzuki, K. Suetsuna, S. Mashiko, T. Nomoto, Y. Toya, B. Yoda, H. Inaba, Chem. Express 1990, 5, 537.
- (b) N. Suzuki, K. Suetsuna, S. Mashiko, B. Yoda, T. Nomoto, Y. Toya, H. Inaba, T. Goto, Agric. Biol. Chem. 1991, 55, 157.
- (a) K. Teranishi, Tokyo Kasei Kogyo Co., Ltd., JP Patent 4453528.
- (b) K. Teranishi, Luminescence 2007, 22, 147.
- 6)Firefly luciferase