Caution Notice:
It has come to our notice that certain fraudulent individuals or entities are misusing our Company’s name and TCI’s registered trademarks by promoting and offering for sale regulated and hazardous chemical substances, including Potassium Cyanide, through online platforms like YouTube. We hereby categorically clarify that TCI has no association or connection whatsoever with the products being displayed or sold in these or similar videos. These products have been falsely represented as being associated with TCI, and the unauthorized use of our trademark and brand name is both illegal and misleading. TCI Chemicals is a responsible global organization committed to adhering to all applicable international and domestic regulatory frameworks. We do not manufacture, distribute, or engage in the sale of regulated chemical substances in India or in any other jurisdiction unless specifically authorized by the relevant laws and regulatory agencies. All our operations and offerings are governed by stringent safety, quality, and compliance protocols in accordance with applicable laws. Further, TCI Chemicals markets and sells its products exclusively through its official website and authorized distributors. We do not sell, endorse, or supply our products through any third-party platforms, unauthorized agents, or resellers. Any individual or organization purchasing products outside these official channels does so at their own risk, and TCI disclaims all responsibility and liability for any consequences arising therefrom. Members of the public, customers, and partners are strongly advised to exercise caution and conduct due diligence before engaging in any transactions involving products claiming association with TCI Chemicals. The official list of our authorized distributors is publicly available and may be accessed at: https://www.tcichemicals.com/IN/en/distributor/index. We are currently pursuing appropriate legal remedies against those misusing our brand, and we reserve all rights available to us under applicable intellectual property and criminal laws. If you become aware of any such fraudulent activity or require clarification, you may reach out to us at: Sales-IN@TCIchemicals.com. Your vigilance and cooperation are essential in safeguarding the public interest and protecting the integrity of the TCI brand.
TCI uses cookies to personalize and improve your user experience. By continuing on our website, you accept the use of cookies. You can change or update your cookiesettings at any time.
Maximum quantity allowed is 999
HPLC Derivatization Reagents
HPLC is used extensively as a means of detecting and determining trace components. In particular, it is effective in the case of non-volatile trace components in complex matrices such as biological substances. Labeling objective substances for analysis with labeling reagents appropriate for detection methods has been performed to obtain higher sensitivity and selectivity. A great number of labeling reagents have been reported for this purpose.
NBD-derivatives and DBD-derivatives developed by Imai, Toyo’oka and co-workers are excellent fluorescent labeling reagents with strong and long wave-length fluorescence resulting from their benzoxadiazole skeleton and they are used for analysis of various biological substances. NBD-Cl was the earliest fluorescence reagent which was applied to HPLC and its effectiveness for the secondary amines, such as proline, was reported. Imai and co-workers developed NBD-F1), in which the chlorine was replaced with fluorine, for HPLC analysis and obtained good results. Also, they developed DBD-F2) where a dimethylsulfamoyl group was introduced into the benzoxadiazole skeleton and used it for the analysis of amino acids by reversed phase HPLC. Because DBD-F itself is non-fluorescent, DBD-labeled amino acids can be detected and analyzed with high sensitivity. DBD-COCl3) reacts with a hydroxy group as well as amines and thiols, and forms a stable fluorescence adduct. In addition, NBD-H and DBD-H4), in which a hydrazino group is introduced into the NBD-skeleton, DBD-skeleton, are used for analysis of carbonyl groups. NBD-PZ and DBD-PZ5), in which a piperazino group is introduced, are used for analysis of carboxyl groups.
For electrochemical detectors (ECD), ferrocene derivatives and NPCA6) have been reported. Ferrocenes have been utilized as the most suitable labeling reagents for ECD because they respond to the application of relatively low voltages, such as +0.5 V, and they are an excellent redox system of oxidation and reduction. NPCA has been developed by the research group of University of Shizuoka recently. NPCA reacts with various primary amines under mild conditions, followed by removal of the THP group to obtain the corresponding α-CA derivatives which have a 6-hydroxychroman skeleton. These derivatives exhibit intense electrochemical activity based on the 6-hydroxychroman skeleton and can be separated efficiently by HPLC on an ODS column.
N-(4-Aminobutyl)-N-ethylisoluminol has been reported to be usable as a chemiluminescence labeling reagent.7)
References
- 1) (a) K. Imai, Y. Watanabe, Anal. Chim. Acta 1981, 130, 377.
- 2) (a) T. Toyo'oka, T. Suzuki, Y. Saito, S. Uzu, K. Imai, Analyst 1989, 114, 413.
- 3) (a) K. Imai, T. Fukushima, H. Yokosu, Biomed. Chromatogr. 1994, 8, 107.
- (b) Tokyo Kasei Kogyo Co., Ltd., Jpn. Kokai Tokkyo Koho 7 238075, 1995.
- 4) S. Uzu, S. Kanda, K. Imai, K. Nakashima, S. Akiyama, Analyst 1990, 115, 1477.
- 5) T. Toyo’oka, M. Ishibashi, Y. Takeda, K. Nakashima, S. Akiyama, S. Uzu, K. Imai, J. Chromatogr. 1991, 588, 61.
- 6) NPCA : Succinimidyl (2R)-6-(Tetrahydro-2H-pyran-2-yloxy)-2,5,7,8-tetramethylchroman-2-carboxylate
- 7) T. Kawasaki, M. Maeda, A. Tsuji, J. Chromatogr. 1985, 328, 121.
Explore HPLC Derivatization Reagents Categories
- Home
- Products
- Analytical Chemistry
- Analytical Reagents
- Derivatization Reagents
- HPLC Derivatization Reagents
- Home
- Products
- Analytical Chemistry
- HPLC
- HPLC Derivatization Reagents