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CAS RN: 10199-89-0 | Product Number: A5592
NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
![Chemical Structure of NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]](/medias/A5592.jpg?context=bWFzdGVyfHJvb3R8NTMwOTJ8aW1hZ2UvanBlZ3xhR0psTDJoa01TODRPVEU1TnpReU1qRTRNamN3TDBFMU5Ua3lMbXB3Wnd8MTgzODA5N2Y2NjUwOGJkYzYyZDYxMGVmZDQ0YTYwZWNlNTNiNGU2NmQxMzYzZDM2OTNhNjBkZjhhZjM1YjM4OQ "NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]")
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![NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]")
Purity: >98.0%(T)
Synonyms:
- 4-Chloro-7-nitrobenzofurazan
- 4-Chloro-7-nitro-2,1,3-benzoxadiazole
Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 1G |
₹2,100.00
|
6 | ≥100 |
|
| 5G |
₹5,400.00
|
3 | ≥100 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
| Product Number | A5592 |
Purity / Analysis Method 
|
>98.0%(T) |
| Molecular Formula / Molecular Weight | C__6H__2ClN__3O__3 = 199.55 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| CAS RN | 10199-89-0 |
| Reaxys Registry Number | 614212 |
| PubChem Substance ID | 87563199 |
| SDBS (AIST Spectral DB) | 5444 |
| MDL Number | MFCD00005808 |
Specifications
| Appearance | Light yellow to Yellow powder to crystal |
| Purity(Volumetric Analysis) | min. 98.0 % |
| Melting point | 97.0 to 100.0 °C |
| Solubility in Methanol | almost transparency |
Properties (reference)
| Melting Point | 98 °C |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
Related Laws:
| RTECS# | DF8002400 |
Transport Information:
Application
HPLC Labeling Reagent for Amines and Thiols
The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with a secondary amine and thiol. The resultant derivative is stable enough to reach the detector without any decomposition under general reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 460 nm and 535 nm, respectively.
Application example:
[Alkylamines] 1)
To 25~500 µL of a methanol solution containing an amine (1~20 µg), 4~8 eq. excess amount of 0.05% labeling reagent 1 / methanol solution is added. After adding 50~100 µL of 0.1 M NaHCO3, incubate at 55 °C for 1~5 h. After cooling the reaction mixture to room temperature, use it as an HPLC sample.
[Others]
TLC and HPLC of N-methylcarbamates, N,N-dimethylcarbamates in agrichemicals2,3) Hydrolyze the carbamates to label the amine derivatives.
TLC of amphetamines in urine4,5), HPLC of prolines (precolumn derivatization method)6)
[Alkylamines] 1)
To 25~500 µL of a methanol solution containing an amine (1~20 µg), 4~8 eq. excess amount of 0.05% labeling reagent 1 / methanol solution is added. After adding 50~100 µL of 0.1 M NaHCO3, incubate at 55 °C for 1~5 h. After cooling the reaction mixture to room temperature, use it as an HPLC sample.
[Others]
TLC and HPLC of N-methylcarbamates, N,N-dimethylcarbamates in agrichemicals2,3) Hydrolyze the carbamates to label the amine derivatives.
TLC of amphetamines in urine4,5), HPLC of prolines (precolumn derivatization method)6)
TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )
HPLC Labeling Reagents ( PDF 10MB )
References
- 1)H.-J. Klimisch, L. Stadler, J. Chromatogr. 1974, 90, 141.
- 2)J. F. Lawrence, R. W. Frei, Anal. Chem. 1972, 44, 2046.
- 3)R. W. Frei, J. F. Lawrence, J. Assoc. Off. Anal. Chem. 1972, 55, 1259.
- 4)J. Monforte, R. J. Bath, I. Sunshine, Clin. Chem. 1972, 18, 1329.
- 5)F. van Hoof, A. Heyndrickx, Anal. Chem. 1974, 46, 286.
- 6)J. H. Wolfram, J. Chromatogr. 1977, 132, 37.
- 7)Y. Nishikawa, K. Kuwata, Anal. Chem. 1985, 57, 1864.
PubMed Literature
Product Articles
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Safety Data Sheet (SDS)
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Specifications
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