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CAS RN: | Product Number: B7077
Biotin Quantitation Kit
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Synonyms:
- Biotin Assay Kit
Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 1KIT |
₹28,500.00
|
Contact Us | 27 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Components : Avidin Buffer 6.25 mL, Biotin Standard (in DMSO) (10mM Biotin in DMSO) 0.1 mL, HABA Buffer 25 mL
- Size : Approx. 34 tests (Cuvette). 170 tests (96-well plate)
Description
- Avidin-biotin binding, the most robust non-covalent protein-ligand interaction in nature, is widely used in biotechnology. When HABA binds to avidin, the resulting complex absorbs light at 500 nm. When a sample containing biotin is added, the HABA is displaced by biotin binding to the avidin. Consequently, the absorbance at 500 nm decreases proportionally to the amount of biotin bound to HABA.
Storage
- Store at 4 ºC. Use within 1 year.
Important Information
- After biotin labeling, remove any unreacted biotinylation reagent from the sample that will be used for biotin quantification using a column or dialysis.
The sample must be dissolved in PBS or TBS for biotin quantification. Avoid buffers containing potassium. If you wish to use a different buffer, compare it with PBS or TBS to ensure there are no issues.
Step-by-Step Protocol
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- B6294 Biotin-C5-Amine (2mg×5)
| Product Number | B7077 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Signal Word | Warning |
| Hazard Statements | H227 : Combustible liquid. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P403 : Store in a well-ventilated place. |
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Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Analytical Charts
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