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Maximum quantity allowed is 999
CAS RN: | Product Number: N1208
Nuclear / Cytoplasmic Fractionation Kit
Purity:
Size | Unit Price | Hyderabad | Japan* | Quantity |
---|---|---|---|---|
1KIT |
₹22,300.00
|
Contact Us | 27 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Components :1×Cytoplasmic Extraction Buffer, 20×Detergent solution, 1×Nuclear Extraction Buffer
- Size : 50 tests
Description
- This product is supplied for fractionating nuclear proteins and cytoplasmic proteins easily from cultured mammalian cells. Extracted protein can be used in downstream applications such as Western blotting.
Directions for Use
- [For Cytoplasmic Extraction]
- 1. Collect cells and wash twice with cold PBS. Use a cell scraper for adherent cells.
- 2. Resuspend the cells gently in cold 1×Cytoplasmic Extraction Buffer. Add 400 μL of the buffer to 100 μL of the cell volume (~ 1 × 107 cells).
- 3. Incubate on ice for 10 minutes.
- 4. Add 20×Detergent solution to the cell suspension and vortex vigorously for 10 seconds. Use 25 μL of the solution per 500 μL of the mixture.
- 5. Centrifuge at 800 × g for 10 minutes at 4 °C.
- 6. Transfer the supernatant (cytoplasmic fraction) to a new tube.
- 7. Resuspend the pellet in cold 1×Nuclear Extraction Buffer. Add 100 μL of the buffer to 100 μL of the pellet volume.
- 8. Incubate on ice for 20 minutes, vortexing every 5 minutes.
- 9. Centrifuge at 15,000 × g for 10 minutes at 4 °C.
- 10. Transfer the supernatant (nuclear fraction) to a new tube.
Storage
- Store at 4 °C.
Related Products
- P2949 100×Protease Inhibitor Cocktail [EDTA free]
- P2976 100×Protease Inhibitor Cocktail
Product Number | N1208 |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Refrigerated (0-10°C) |
Condition to Avoid | Heat Sensitive |
Articles/Brochures
Safety Data Sheet (SDS)
The requested SDS is not available.
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Specifications
C of A & Other Certificates
Sample C of A
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Analytical Charts
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