iCBioflim-H1 (Product No. 4031) is applicable to clearing many kinds of biofilms. If the transparency with iCBiofilm-H1 is insufficient, iCBiofilm-H2 (Product No. T4032) can be used instead. Since iCBiofilm-H2 is prepared at high component concentration, iCBiofilm-H2 is available as a stock solution. Before addition to biofilm, iCBiofilm-H2 has to be diluted with deionized water to the appropriate concentration. (Undiluted iCBiofilm-H2 may work well.)
[Direction for Use]
Prepare a biofilm in a glass bottomed culture dish (35mm diam.).
After removing planktonic cells and the culture medium from the dish, add 1% glutaraldehyde solution diluted with PBS to fix the biofilm at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, the use of 4% paraformaldehyde in PBS is recommended.
Remove the glutaraldehyde solution and wash three times with PBS or distilled water.
Stain the biofilm with appropriate fluorescence reagents.
Remove staining reagents and add iCBiofilm solution.