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CAS RN: | Product Number: A2659

AOL-Biotin Conjugate


Purity:
Synonyms:
  • Aspergillus oryzae lectin (= AOL)-Biotin Conjugate
  • AOL-biotin [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
Documents:
1ML
$430.00
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Note:

Product Information

Quantity : 1 ml per vial (1 mg/1 ml: Lowry method)
Product Form : Biotin-immobilized recombinant AOL, Liquid
Buffer : 50 mM Phosphate buffer, pH6.5
Molecular Mass : >35 kDa
Preservative : None
Stabilizer : None

Description

The AOL is a carbohydrate-binding protein (Lectin) derived from Aspergillus oryzae. The AOL gene product is expressed in Aspergillus oryzae. (The gene is derived from the same origin as the host cell.) This lectin preferably binds to a [Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)] glycan epitopes.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The AOL-Biotin Conjugate shows significant interaction with human lactoferrin (hLF) possessing [Fucα(1-6)/α(1-3)] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the AOL-Biotin Conjugate specifically binds to α(1-6/1-3)Fucsylated hLF but not to unfucosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B.


Storage

Store at 2-10°C.


Related Products

L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5 mg/ml, PBS pH6.5)
A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]

Product Number A2659
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Light yellow clear liquid
Concentration(Lowry method) 0.9 to 1.5 mg/mL
Molecular weight 30 to 40 kDa
Suitability for use in ELISA tests min. 1.0 (human Lactoferrin, 10 micro g/mL, OD450)
Fucose inhibition test to pass test
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 0511.99.4070
Application
Fucose-specific lectin

References


Application
Biotinylated Fucose-specific Lectin

References


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