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CAS RN: 23491-45-4 | Product Number: B6236

Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]


Purity:
Synonyms:
  • 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride (0.2mL×5) (1mg/mL in Water)
  • 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-[2,5'-bibenzimidazol]-2'-yl]phenol Trihydrochloride (0.2mL×5) (1mg/mL in Water)
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$41.00
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Note:

Product Information
Volume : 0.2 mL x 5 vial
Concentration : 1.0 mg/mL

Description
Bisbenzimide H 33258 is a fluorescent stain for labeling DNA.
DNA-bound Bisbenzimide H 33258 is readily excited with UV and emits fluorescence in the blue region of the visible light spectrum (Excitation : 355 nm / Emission : 465 nm).

Description for Use
Fluorescence Staining of Cell Nucleus
Fix and permeabilize cells as desired.
Dilute Bisbenzimide H 33258 solution 1/1000* with PBS or an appropriate buffer.
Incubate with cells at room temperature for 5-20 minutes**.
*Typical working concentration used in most applications is 0.1μg/mL-10μg/mL.
Incubate with cells at room temperature for 5-30 minutes**.
** Extend Incubate time as desired.
Mount sample with an appropriate medium and proceed to imaging.

Storage
Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

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Product Number B6236
Molecular Formula / Molecular Weight C__2__5H__2__4N__6O·3HCl = 533.88 
Physical State (20 deg.C) Liquid
Storage Temperature Frozen (<0°C)
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 23491-45-4
Reaxys Registry Number 4088183
PubChem Substance ID 468590991
MDL Number

MFCD00012679

Specifications
Appearance Light yellow to Yellow clear liquid
DNA staining test pass test
Properties (reference)
Boiling Point 100 °C
GHS
Related Laws:
RTECS# SM1140500
Transport Information:
HS Number 2934.99.4400
TCIMAIL
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