Maximum quantity allowed is 999
Please select the quantity
CAS RN: 23491-45-4 | Product Number: H1343
Bisbenzimide H 33258 Hydrate [for Biochemical Research]
×
![]()
×
![Bisbenzimide H 33258 Hydrate [for Biochemical Research]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "Bisbenzimide H 33258 Hydrate [for Biochemical Research]")
Purity: >98.0%(HPLC)
Synonyms:
- 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate
Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 25MG |
$64.00
|
Contact Us | Contact Us | ≥20 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
| Product Number | H1343 |
| Purity / Analysis Method | >98.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__2__5H__2__4N__6O·3HCl·xH__2O = 533.88 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | <0°C |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 23491-45-4 |
| Reaxys Registry Number | 4088183 |
| PubChem Substance ID | 172089156 |
| MDL Number | MFCD00012679 |
Specifications
| Appearance | Light yellow to Brown to Dark green powder to crystal |
| Purity(HPLC) | min. 98.0 area% |
| Water(Vaporization methoed) | max. 20.0 % |
| DNA staining test | to pass test |
Properties (reference)
| Melting Point | 280 °C |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P302 + P352 : IF ON SKIN: Wash with plenty of soap and water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P362 : Take off contaminated clothing and wash before reuse. |
Related Laws:
| RTECS# | SM1140500 |
Transport Information:
| HS Number | 2933.99.8290 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
Application
Fluorometric Assays of DNA Using Bisbenzimide H 33258
References
- A simple, rapid, and sensitive DNA assay procedure
- Fluorometric assay of DNA in cartilage explants using Hoechst 33258
- Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye
- DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water
- Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG)2 duplex: calorimetric and spectroscopic studies
- Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study
PubMed Literature
Articles/Brochures
TCIMAIL
Documents
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number is entered
Analytical Charts
Please enter Lot Number
Incorrect Lot Number is entered
The requested Analytical Chart is not available.