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CAS RN: 23491-45-4 | Product Number: H1343

Bisbenzimide H 33258 Hydrate [for Biochemical Research]

Purity: >98.0%(HPLC)
  • 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate
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Product Number H1343
Purity / Analysis Method >98.0%(HPLC)
Molecular Formula / Molecular Weight C__2__5H__2__4N__6O·3HCl·xH__2O = 533.88  
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 23491-45-4
Reaxys Registry Number 4088183
PubChem Substance ID 172089156
MDL Number


Appearance Light yellow to Brown to Dark green powder to crystal
Purity(HPLC) min. 98.0 area%
Water(Vaporization methoed) max. 20.0 %
DNA staining test to pass test
Properties (reference)
Melting Point 280 °C
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
RTECS# SM1140500
Transport Information:
HS Number 2933.99.8290
Immunofluorescence Staining

The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL (shown in red
   Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL (shown in green
   Hoechst 33258 [H1343] 1 µg/mL(shown in blue

Fluorometric Assays of DNA Using Bisbenzimide H 33258


PubMed Literature

Safety Data Sheet (SDS)
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