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CAS RN: 23491-45-4 | Product Number: H1343
Bisbenzimide H 33258 [for Biochemical Research]
![Chemical Structure of Bisbenzimide H 33258 [for Biochemical Research]](/medias/Tci-440-H1343.jpg?context=bWFzdGVyfHJvb3R8MTg2NDB8aW1hZ2UvanBlZ3xhR1kwTDJneFpTODVORFUyTmpjMU5qRTRPRFEyTDFSamFTMDBOREJmU0RFek5ETXVhbkJufDFiMDQ3ZTI0MGI4ZjQxMGE1MWE3OTYxNjM5NzEwNjA3OTQxNmFjY2ZkY2EwNjY1NmU5NjFjNGI4YjI3NWQzMDE "Bisbenzimide H 33258 [for Biochemical Research]")
![Thumbnail of Bisbenzimide H 33258 [for Biochemical Research]](/medias/Tci-98-H1343.jpg?context=bWFzdGVyfHJvb3R8MjU5NnxpbWFnZS9qcGVnfGFETXpMMmd4WlM4NU5EVTJOamMxTmpnME16Z3lMMVJqYVMwNU9GOUlNVE0wTXk1cWNHY3w3ZTBiY2FhYzNmYTNhYWQ4OTk3ZWMzOGRhNGRlNjMwNDExM2E5ZmI3NGZiZmNkNWFmZTZmZjcwYjdmZGEzY2Rl)
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Purity: >98.0%(HPLC)
Synonyms:
- 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 25MG |
$65.00
|
1 | 1 | 8 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Previously this product had been labeled as a hydrate form both in product name and molecular formula. The hydrate form had no exclusive CAS RN and used to share the same CAS RN as the anhydrous form.
To avoid any misleading information and since moisture in product is considered as an impurity, we have decided to remove the term 'Hydrate' from product name and 'xH2O' from the molecular formula for such products.
Please use it at ease since there is no difference in product quality, even if you see the previous indications on a part of label.
| Product Number | H1343 |
Purity / Analysis Method 
|
>98.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__2__5H__2__4N__6O·3HCl = 533.88 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 23491-45-4 |
| Related CAS RN
|
2138819-37-9 |
| Reaxys Registry Number | 4088183 |
| PubChem Substance ID | 172089156 |
| MDL Number | MFCD00012679 |
Specifications
| Appearance | Light yellow to Brown to Dark green powder to crystal |
| Purity(HPLC) | min. 98.0 area% |
| Water(Vaporization method) | max. 20.0 % |
| DNA staining test | to pass test |
Properties (reference)
| Melting Point | 280 °C |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P302 + P352 : IF ON SKIN: Wash with plenty of soap and water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P362 : Take off contaminated clothing and wash before reuse. |
Related Laws:
| RTECS# | SM1140500 |
Transport Information:
| HS Number | 2933.99.7900 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
Application
Fluorometric Assays of DNA Using Bisbenzimide H 33258
References
- A simple, rapid, and sensitive DNA assay procedure
- Fluorometric assay of DNA in cartilage explants using Hoechst 33258
- Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye
- DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water
- Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG)2 duplex: calorimetric and spectroscopic studies
- Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study
PubMed Literature
Documents
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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Analytical Charts
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Other Documents
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![Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/B6236.jpg?context=bWFzdGVyfHJvb3R8MTc2MTF8aW1hZ2UvanBlZ3xhREE1TDJnNU15ODVNVFF3T0RjNE1URTBPRFEyTDBJMk1qTTJMbXB3Wnd8MWEwZWI2OGMxZjUwNmMzYzU2ODU5MjA2MjNkZjk5MTliMGM3MGQ2MmVhNjFjN2I1ZDcxNTcwODcwZTBhNGFmYw "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
| Product # | B6236 |
| CAS RN | 23491-45-4 |
| Purity / Analysis Method: |